胚胎移植操作对宫腔中胚胎运动、沉积的影响  

Embryo transfer parameters effects on the movement and deposition of embryo in the uterine cavity

作　　者：杨青 陶帅 牛志宏[2] 莫锦秋[1] Yang Qing;Tao Shuai;Niu Zhi-hong;Mo Jin-qiu(School of Mechanical Engineering,Shanghai Jiao Tong University,Shanghai 200240,China;Reproductive Medicine Center,Ruijin Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China)

机构地区：[1]上海交通大学机械与动力工程学院,上海市200240 [2]上海交通大学医学院附属瑞金医院生殖医学中心,上海市200025

出　　处：《中国组织工程研究》2018年第24期3880-3887,共8页Chinese Journal of Tissue Engineering Research

基　　金：上海交通大学医工交叉研究基金面上项目(体外胚胎移植模拟系统在改善IVF妊娠结局中的应用)(2014年)~~

摘　　要：背景:目前胚胎种植率维持在20%-30%,即移植入子宫的胚胎有70%-80%未能着床。除了胚胎质量、子宫内膜容受性以外,恰当的胚胎移植操作也是成功着床的重要因素。目的:通过数值仿真和体外胚胎移植实验,研究不同胚胎移植参数配合下人类胚胎进入宫腔后的沉积位置,得到最佳胚胎移植参数配合。方法:以3个参数即注射时间(T)、移植导管端部与宫底距离(L)、子宫角度(A)的组合进行实验。其中注射时间取值10,20,30 s,移植导管端部与宫底距离取值5,10,15 mm,子宫角度取值-60°、-45°,-30°,-15°,0°,15°,30°,45°,60°。结果与结论:(1)比较了几种胚胎移植液体积对最终沉积位置的影响,根据最终分析选择了60μL作为胚胎移植液的体积;(2)不同子宫角度时,颗粒的运动轨迹和最终沉积位置的原因是由于不同角度导致重力不同,最佳子宫角度区间为15°-30°;(3)对比3种注射时间对最终沉积位置的影响,最佳注射时间为10 s;(4)对比3种移植管端部与宫底距离对最终沉积位置的影响,最佳移植管端部与宫底距离为10 mm。BACKGROUND： The rate of embryo implantation is at 20%-30%, suggesting that 70%-80% of the embryos fail in implantation. Except quality of embryo and endometrial receptivity, proper embryo transfer is also an important factor in success. OBJECTIVE： To investigate the sedimentary position of human embryo into the uterine cavity with different embryo transfer parameters through numerical simulation and in vitro embryo transfer experiment. METHODS： The combination of 3 parameters including injection time（10, 20, 30 seconds）, distance between transfer tube end and bottom of the uterus（5,10, 15 mm）, and the uterus angle（-60°,-45°,-30°,-15°, 0°, 15°, 30°, 45°, 60°）. RESULTS AND CONCLUSION： After comparative analysis, 60 μL was selected as the volume of embryo transfer fluid. At different angles of the uterus, the changes in the trajectories of particles and the final sedimentary position were caused by gravity, and the optimal angle was in 15°-30°. Additionally, the optimal injection time was 10 seconds, and the distance between transfer tube end and bottom of the uterus was 10 mm.

关 键 词：胚胎移植 着床率 注射时间 子宫角度 移植导管端部与宫底距离 沉积位置 运动轨迹 最佳参数 组织构建 

分 类 号：R318[医药卫生—生物医学工程]