B群链球菌鉴别培养基的研究  

Study on the indicator of culture-medium for group B Streptococcus

作　　者：金明哲[1] 吴湘玉 Jin Mingzhe,Wu Xiangyu(Department of Pathogenic Biology,Zhuhai Campus of Zunyi Medical University,Zhuhai Guangdong 519041,China)

机构地区：[1]遵义医学院珠海校区病原生物学教研室,广东珠海519041

出　　处：《遵义医学院学报》2018年第3期277-281,共5页Journal of Zunyi Medical University

摘　　要：目的研究B群链球菌(group B streptococcus,GBS)鉴别培养基的最佳配方和鉴别培养效果。方法通过观察GBS在不同配方培养基上的生长状况及显色反应,筛选确定GBS鉴别培养基的最佳配方;在最佳配方条件下引入直肠和生殖道中常见的其他杂菌进行干扰试验,进一步检测GBS鉴别培养基的鉴别作用。结果当可溶性淀粉浓度为1.0%~2.5%,血清浓度≥7.0%,p H为7.2~7.7时,GBS鉴别培养基上的B群链球菌显色反应最明显,且其鉴别作用不受其他杂菌影响。结论 GBS鉴别培养基适用于GBS的鉴别培养。Objective To study the best formula for group B streptococcus（ GBS） indicator in culture-medium and its effectiveness of differential culture. Methods By observing the colonies development and chromogenic reaction of GBS on the medium with different formula,the best formula for GBS indicator culture-medium was selected. The interference test was carried out with other common bacteria in the rectum and genital tract to further determine the effectiveness of the GBS indicator culture-medium. Results When the concentration of soluble starch was 1% ～ 2. 5%,the concentration of serum was more than 7%,and p H value was between 7. 2 and 7. 7,the chromogenic reaction of GBS on the indicator culture-medium was most significant,and its differential function was not affected by other bacteria. Conclusion GBS indicator culture-medium is suitable for differential culture of GBS.

关 键 词：GBS 鉴别培养基 最佳配方 干扰试验 

分 类 号：R378.12[医药卫生—病原生物学]