芽孢杆菌C412的廉价培养基优化及其亚甲基蓝去除的研究  

Optimization of inexpensive medium and exploration of removing methylene blue by Bacillus sp. C412

作　　者：韩鹤松 张子莹 王浩 肖冬梅 周倩 刘海波[1] HAN He-song;ZHANG Zi-ying;WANG Hao;XIAO Dong-mei;ZHOU Qian;LIU Hai-bo(School of Chemistry and Chemical Engineering,Guangxi University,Nanning 530004,China)

机构地区：[1]广西大学化学化工学院,广西南宁530004

出　　处：《广西大学学报（自然科学版）》2018年第3期1277-1285,共9页Journal of Guangxi University（Natural Science Edition）

基　　金：国家自然科学基金资助项目(31360020;31560014);广西大学科研基金资助项目(XGZ130080)

摘　　要：为研究芽孢杆菌C412去除亚甲基蓝的能力,首先通过实验得到了一个廉价培养基配方:糖蜜废液含量5%、2.5 g/L糖蜜、1 g/L牛肉膏、3 g/L(NH_4)_2SO_4、1 g/L NaCl、0.05 g/L MgSO_4·7H_2O、5 g/L K_2HPO_4、2 g/L KH_2PO_4(pH=7),在该培养基条件下,可以将目标细菌C412培养17 h后得到最大生长量OD_(600)为3.12的发酵液。将培养出来的细菌,通过亚甲基蓝的去除条件优化实验,得到了一个最佳的亚甲基蓝去除条件:亚甲基蓝初始浓度为25 mg/L,发酵体系pH为7.63,初始菌密度OD_(600)为0.18,发酵时间为18 h,在该条件下亚甲基蓝去除率可以达到96.77%。研究结果表明芽孢杆菌C412具有很好的亚甲基蓝去除能力,工业化应用前景广阔。In this study,cultivation of C412 strain was achieved using an optimized culture medium containing（ per liter） 5% molasses waste water,2. 5 g/L molasses,1 g/L beef extract,3 g/L（ NH_4）_2SO_4,1 g/L Na Cl,5 g/L K_2HPO_4,2 g/L KH_2PO_4,0. 05 g/L MgSO_4·7 H_2O（ pH = 7）.Under this condition,the OD_（600） of C412 could reach 3. 12 after 17 h. Furthermore C412 was used to study the optimal condition for removing methylene blue, and the highest removal efficiency（ 97. 09%） was achieved with following conditions： the concentration of methylene blue 25 mg/L,the OD_（600） of the initial cell inoculation dosage 0. 18,pH 7. 6 and fermentation time 18 h. These results showed that Bacillus sp. C412 was capable of removing methylene blue efficiently and had abright future in industrial application.

关 键 词：廉价培养基 糖蜜废液 亚甲基蓝 

分 类 号：TQ46[化学工程—制药化工]