弹性蛋白及弹性蛋白降解产物在肝硬化患者中的表达特点  

Characteristics of elastin and elastin derived peptides in patients with cirrhosis

作　　者：杨爱婷[1] 赵文姗[1] 张允[1] 王欢[1] 尤红[1] YANG Ai-ting;ZHAO Wen-shan;ZHANG Yun(Experimental and Translational Research Center,Beijing Friendship Hospital,Capital Medical University; Beijing Clinical Institute; Biobank ofClinical Resources; Beijing Key Laboratory of Tolerance Induction and Organ Protection in Transplantation,National Clinical Research Center of Digestive Diseases,Beijing 100050,China)

机构地区：[1]首都医科大学附属北京友谊医院科研实验中心北京市临床医学研究所临床数据与样本资源库移植耐受与器官保护北京市重点实验室国家消化系统疾病临床医学研究中心,北京100050

出　　处：《临床和实验医学杂志》2018年第13期1345-1347,共3页Journal of Clinical and Experimental Medicine

基　　金：国家自然科学基金资助项目(编号:81500456);中国肝炎防治基金会王宝恩肝纤维化研究基金资助项目(编号:20151009)

摘　　要：目的建立并优化竞争法酶联免疫吸附试验(ELISA)对弹性蛋白降解产物(EDP)的检测;观察弹性蛋白和EDP在肝硬化患者中的表达特点。方法回顾性纳入失代偿期肝硬化患者14例,健康对照人群10例,应用竞争法ELISA检测两组人群EDP表达水平,应用商品化ELISA试剂盒检测两组人群弹性蛋白水平。根据建立的剂量-反应曲线和曲线拟合的程度,选择最佳的竞争法ELISA检测EDP的条件。结果成功建立并优化竞争法ELISA检测EDP的条件,标准曲线呈线性拟合,R^2=0.99。通过对弹性蛋白和EDP的检测发现,肝硬化患者弹性蛋白的水平显著高于健康对照组[(32.19±12.17)ng/ml vs.(1.90±0.46)ng/ml,P<0.05)],而EDP的表达显著低于健康对照组[(28.20±1.80)μg/ml vs.(60.80±1.46)μg/ml,P<0.05]。结论弹性蛋白和EDP水平与失代偿期肝硬化相关。Objective To establish and optimize the method of competitive enzyme-linked immunosorbent assay（ ELISA） for elastin derived peptides（ EDP）,meanwhile to observe the expression of elastin and EDP in patients with cirrhosis. Methods According to the establishment of dose-response curve,the competitive ELISA for EDP were optimized. This is a retrospective study,which were recruited 14 cases of decompensated liver cirrhosis patients and 10 cases in healthy control group. Expression of elastin and EDP were measured using competitive ELISA and commercial ELISA kit respectively. Results The competitive ELISA for EDP were established and optimized successfully. The standard curves were linear fitted and R^2= 0. 99. The expression of elastin in cirrhotic patients was significantly higher than healthy control group [（ 32. 19± 12. 17） ng/ml vs（ 1. 90 ± 0. 46） ng/ml,P〈0. 05],meanwhile EDP expression was significantly lower than healthy control group [（ 28. 20 ±1. 80） μg/ml vs（ 60. 80 ± 1. 46） μg/ml,P〈0. 05]. Conclusion These findings suggest that levels of elastin and EDP are associated with the development of decompensated liver cirrhosis.

关 键 词：肝纤维化 肝硬化 弹性蛋白 弹性蛋白降解产物 酶联免疫吸附试验 

分 类 号：R446.1[医药卫生—诊断学] R575.2[医药卫生—临床医学]