妊娠期糖尿病脂肪细胞转染网膜素1对葡萄糖摄取率的影响及MEK/ERK通路蛋白的参与研究  

The effect of omentin 1 transfection on glucose uptake and the involvement of MEK/ERK pathway protein in gestational diabetes adipose cells

作　　者：陈曼诗[1] 程丽琴[1] 付艳霞[1] 盛丛 CHEN Man-shi, CHENG Li-qin, FU Yan-xia, et al.(Department of Obstetrics and Gynecology, The Eighth Hospital of Sun Yat-sen University, Shenzhen Guangdong 518000, China)

机构地区：[1]中山大学附属第八医院妇产科,广东深圳518000

出　　处：《临床和实验医学杂志》2018年第14期1504-1508,共5页Journal of Clinical and Experimental Medicine

摘　　要：目的分析妊娠期糖尿病脂肪细胞转染网膜素1对葡萄糖摄取率的影响及MEK/ERK通路蛋白的参与作用。方法构建网膜素1过表达载体转染传代脂肪细胞,分析不同转染浓度(0μg、1.0μg、2.0μg、4.0μg)脂肪细胞的葡萄糖摄取率。采用荧光定量PCR法、Western Blotting法和免疫细胞化学法分析MEK/ERK通路蛋白的表达变化。结果不同网膜素1转染浓度对脂肪细胞葡萄糖摄取率和MEK/ERK通路蛋白及IRS水平有明显影响,且随着网膜素1转染浓度的升高,葡萄糖摄取率逐渐升高(P<0.05),MEK、ERK1/2蛋白(P<0.05)、mRNA(P<0.05)水平逐步降低,而IRS-1蛋白水平明显升高(P<0.05)。结论妊娠期糖尿病脂肪细胞转染网膜素1后细胞的葡萄糖摄取率及IRS明显升高而MEK/ERK通路蛋白表达降低,表明网膜素1过表达和MEK/ERK通路蛋白被抑制与葡萄糖葡萄糖摄取和胰岛素抵抗密切相关。Ojective In this study, the effect of omentin 1 on glucose uptake and the involvement of MEK/ERK pathway protein in gestational diabetes adipose cells were analyzed. Methods In this paper, omentin 1 overexpression vector was constructed to transfect adipocytes, and the glucose uptake rate of adipocytes at different transfection concentrations （0 μg, 1 μg, 2 μg, 4 μg） was analyzed. The expression of MEK/ERK pathway protein was analyzed by fluorescence quantitative PCR, Western Blotting and immunocytochemistry. Results The concentration of omentin 1 transfected significantly affected the glucose uptake rate and MEK/ERK pathway protein and IRS level of adipocytes. The glucose uptake rate increased gradually with the increase of omentin 1 transfection concentration （ P 〈0.05）. The level of MEK, ERK1/2 protein （ P 〈0.05） and mRNA （ P 〈0.05） decreased gradually and the level of IRS-1 protein increased significantly （ P 〈0.05）. Conclusion After transfection of omentin 1, the glucose uptake rate and IRS increased significantly, while the expression of MEK/ERK pathway protein decreased. The expression of omentin 1 and the inhibition of MEK/ERK pathway protein were closely related to glucose glucose uptake and insulin resistance.

关 键 词：妊娠期糖尿病 脂肪细胞 转染 网膜素1 葡萄糖摄取率 MEK/ERK通路 

分 类 号：R714.256[医药卫生—妇产科学]