黄芪百合颗粒对亚硝酸钠中毒致缺氧性损伤小鼠的保护作用  

Protective effects of Astragalus Lily Granules on sodium nitrite-induced anoxic injury in mice

作　　者：张苡铭 苏韫 颜春鲁 刘永琦 张丽昕[1] 陈刚 冯彩琴 成新平 ZHANG Yi-ming;SU Yun;YAN Chun-lu;LIU Yong-qi;ZHANG Li-xin;CHEN Gang;FENG Cai-qin;CHENG Xin-ping(,Provincial-level Key Laboratory for Molecular Medicine of Major Diseases and the Prevention and Treatment with Traditional Chinese Medicine Research in Gansu Colleges and Universities,Gansu University of Traditional Chinese Medicine,,Lanzhou 730000,China;Ministry of Education Key Laboratory for Dunhuang Medicine and Transformation Co-founded by Gansu Province and MOST,Lanzhou 730000,China)

机构地区：[1]甘肃中医药大学甘肃省高校重大疾病分子医学与中医药防治研究省级重点实验室,甘肃兰州730000 [2]敦煌医学与转化省部共建教育部重点实验室,甘肃兰州730000

出　　处：《中成药》2018年第7期1479-1483,共5页Chinese Traditional Patent Medicine

基　　金：陇原青年创新人才扶持计划(2014-93-72);甘肃省高校重大疾病分子医学与中医药防治重点实验室开放基金(ZYX15-16-3);2014年省级自然科学基金B类计划(148RJZA080)

摘　　要：目的观察黄芪百合颗粒对亚硝酸钠致缺氧性损伤小鼠的保护作用。方法 72只小鼠随机分为空白组,阳性对照组,模型组,黄芪百合颗粒低、中、高剂量组(1.75、3.5、7 mg/kg),各实验组小鼠灌胃给予相应药物30 d。末次给药1 h后,各组(除空白组外)小鼠腹腔注射亚硝酸钠(100 mg/kg),1 h后采血,比色法测定高铁血红蛋白(Met Hb)含有量,ELISA法测定硫代巴比妥酸反应物(TBARS)含有量,HE染色观察小肠病理学变化,免疫组织化学法检查脑组织中脑红蛋白(Ngb)表达。结果与空白组比较,模型组Met Hb、TBARS含有量显著提高(P<0.01),小肠黏膜上皮大量脱落,炎细胞浸润数量、Ngb表达显著增加(P<0.01);与模型组比较,黄芪百合颗粒各剂量组和阳性对照组Met Hb、TBARS含有量显著降低(P<0.05,P<0.01),小肠黏膜上皮少量脱落,炎细胞浸润数量显著减少(P<0.05,P<0.01),Ngb表达显著增加(P<0.01),胞浆、胞核均可见棕黄色颗粒状免疫反应阳性产物。结论黄芪百合颗粒可通过降低Met Hb含有量、抑制膜脂过氧化水平、改善小肠病理程度、提高Ngb表达来显示对亚硝酸钠致缺氧性损伤小鼠的保护作用。AIM To observe the protective effects of Astragalus Lily Granules（ ALG） on sodium nitrite-induced anoxic injury in mice. METHODS Seventy-two mice were randomly divided into blank group,positive control group,model group,low-dose ALG group（ 1. 75 mg/kg）,medium-dose ALG group（ 3. 5 mg/kg） and high-dose ALG group（7 mg/kg）,the mice in various experimental groups were given 30-day intragastric administration of corresponding drugs. After 1 h of last administration,the mice in the various groups（ except for the blank group） were intraperitoneally injected with sodium nitrite（ 100 mg/kg）,followed by blood sampling after 1 h.Methemoglobin（ Met Hb） content was determined by colorimetric method,thiobarbituric acid reactive substance（TBARS） content was determined by ELISA,small intestinal pathological changes were observed by HE staining,and neuroglobin（ Ngb） expression was detected by immunohistochemical method. RESULTS Compared with the blank group,the model group manifested with significantly increased Met Hb and TBARS contents（ P 〈 0. 01）,small intestinal mucosal epithelium’s much abscission,significantly increased inflammatory cell infiltration number and Ngb expression（ P 〈 0. 01）. Compared with the model group,the ALG groups and the positive control group shared decreased Met Hb and TBARS contents（ P 〈 0. 05,P 〈 0. 01）,small intestinal mucosal epithelium’ s little abscission,significantly decreased inflammatory cell infiltration number（ P 〈 0. 05,P 〈 0. 01）,significantly increased Ngb expression（ P 〈 0. 01）,and brown-yellow granular positive immune response products were observable in cytoplasm and nucleus. CONCLUSION ALG can exhibit protective effects on sodium nitrite-induced anoxic injury in mice by decreasing Met Hb content,inhibiting membrane lipid peroxidation level,improving small intestinal pathological degree,and increasing Ngb expression.

关 键 词：黄芪百合颗粒 缺氧性损伤 亚硝酸钠中毒 

分 类 号：R285.5[医药卫生—中药学]