HPLC法同时测定化瘀祛斑胶囊中4种成分  

Simultaneous determination of four constituents in Huayu Quban Capsules by HPLC

作　　者：董虹玲 李钦青 柴金苗 贺文彬 李秀英 DONG Hong-ling;LI Qin-qing;CHAI Jin-miao;HE Wen-bin;LI Xiu-ying(Shanxi University of Chinese Medicine,Jinzhong 030619,China)

机构地区：[1]山西中医药大学,山西晋中030619

出　　处：《中成药》2018年第7期1519-1522,共4页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金资助项目(81681260247);山西省科技创新团队建设计划项目(2013131022)

摘　　要：目的建立HPLC法同时测定化瘀祛斑胶囊(柴胡、薄荷、黄芩等)中4种成分的含有量。方法该药物50%甲醇提取液的分析采用Waters C_(18)色谱柱(4.6 mm×250 mm,5μm);流动相乙腈-0.2%磷酸,梯度洗脱;检测波长232 nm;体积流量1.0 mL/min;柱温30℃。结果羟基红花黄色素A、芍药苷、黄芩苷、蒙花苷分别在0.760 8~8.368 8、6.16~67.76、32.136~353.496、1.454 4~15.998 4μg/mL范围内呈良好的线性关系(r≥0.999 5),平均加样回收率分别为100.1%、99.52%、99.93%、100.25%,RSD分别为1.40%、1.14%、1.70%、2.63%。结论该方法稳定可靠,可用于化瘀祛斑胶囊的质量控制。AIM To establish an HPLC method for the simultaneous content determination of four constituents in Huayu Quban Capsules（ Bupleuri Radix,Menthae haplocalycis Herba,Scutellariae Radix,etc.）. METHODS The analysis of 50% methanol extract of this drug was performed on a 30 ℃ thermostatic Waters C18 column（4. 6 mm × 250 mm,5 μm）,with the mobile phase comprising of acetonitrile-0. 2% phosphoric acid flowing at 1. 0 m L/min in a gradient elution manner,and the detection wavelength was set at 232 nm. RESULTS Hydroxy safflower yellow A,paeoniflorin,baicalin,linarin showed good linear relationships within the ranges of 0. 760 8-8. 368 8,6. 16-67. 76,32. 136-353. 496,1. 454 4-15. 998 4 μg/m L（ r≥0. 999 5）,whose average recoveries were 100. 1%,99. 52%,99. 93%,100. 25% with the RSDs of 1. 40%,1. 14%,1. 70%,2. 63%,respectively. CONCLUSION This stable and reliable method can be used for quality control of Huayu Quban Capsules.

关 键 词：化瘀祛斑胶囊 羟基红花黄色素A 芍药苷 黄芩苷 蒙花苷 HPLC 

分 类 号：R927.2[医药卫生—药学]