初步探讨等量稀释法解决黄疸标本引起的LH750仪白细胞无法分类的问题  

Preliminary discussion on using equal dilution to solve the problem that LH750 could not classify white blood cells caused by Jaundice specimen

作　　者：陈敬潮 周东铭 连斯敏 张苏伟[1] CHEN Jingchao;ZHOU Dongming;LIAN Simin;ZHANG Suwei(Department of Clinical Laboratory,Shantou Central Hospital in Guangdong Province,Shantou 515041,China)

机构地区：[1]广东省汕头市中心医院检验科,广东汕头515041

出　　处：《中国现代医生》2018年第13期111-114,169,共5页China Modern Doctor

摘　　要：目的探讨血液细胞分析仪无法分类的黄疸标本,通过生理盐水等量稀释后其白细胞分类结果准确性和可靠性。方法将无白细胞分类计数结果的黄疸标本与生理盐水等倍稀释后在LH750仪上机进行检测,将稀释后的白细胞分类计数结果与原血液标本在XN-1000血细胞分析仪的计数结果做对比,分析稀释后的黄疸标本的白细胞分类结果与原血液标本白细胞分类是否有差异。结果生理盐水稀释的黄疸标本,WBC<2.0×10~9/L时,相对偏差最小为0%,最大为9.03%;WBC>2.0×10~9/L时,相对偏差最小为0.09%,最大为5.21%,均符合要求。结论生理盐水等量稀释法可用于初步解决黄疸标本白细胞无法分类计数的问题。Objective To investigate the accuracy and reliability of white blood cell classifications in normal saline diluted jaundice specimen which could not be classified by blood cell analyzer. Methods Jaundice specimens which not be classified were equally diluted with normal saline and assessed in LH750. Differential count of white blood cells after dilution was compared with the result of original specimen using XN-1000 blood cell analyzer. The white blood cell classifications of diluted jaundice specimen and the original blood sample were analyzed. Results In jaundice specimens diluted by normal saline, when WBC 2.0 ×109/L, minimum relative deviation was 0% and the maximum was9.03%. When WBC〉2.0×109/L, minimum relative deviation was 0.09% and the maximum was 5.21%. All the results met the requirements. Conclusion Equal dilution of normal saline could preliminarily solve the problem that white blood cells could not be classified in jaundice specimen.

关 键 词：LH750血细胞分析仪 白细胞五分类 比对试验 高胆红素 

分 类 号：R446.111[医药卫生—诊断学]