基于转录组数据的银鲴微卫星位点筛选  

Screening of microsatellite markers in Xenocypris argentea using transcriptome sequencing

作　　者：赵良杰 彭新亮 郭旭升 ZHAO Liang-jie;PENG Xin-liang;GUO Xu-sheng(Xinyang Agriculture and Forestry University/Fishery Biological Engineering Technology Research Center of Henan Province,Xinyang 464000,Henan,China)

机构地区：[1]信阳农林学院水产学院,河南省渔业生物工程技术研究中心,河南信阳464000

出　　处：《淡水渔业》2018年第4期23-29,共7页Freshwater Fisheries

基　　金：河南省科技攻关项目(162102110053)

摘　　要：采用Illumina高通量测序技术对银鲴(Xenocypris argentea)肝脏组织进行转录组测序分析,筛选微卫星标记并进行多态性检验。共获得7 272个微卫星位点。随机选取48个4碱基以上重复类型的SSR位点进行引物设计和PCR验证,有47对可以扩增出清晰稳定的条带。在上述47个位点中随机选择26对在洞庭湖银鲴群体中进行多态性分析,结果表明,具有多态性的微卫星引物为21对。不同多态位点得到的等位基因数范围为3~15,平均基因数为7.29±3.94,观测杂合度(Ho)、期望杂合度(He)、多态信息含量(PIC)以及香浓-威尔指数(H)平均值分别为0.532 7±0.211 4、0.613 6±0.196 4、0.564 6±0.198 0和1.302 0±0.577 9。21个微卫星位点中,有6个显著偏离哈代-温伯格平衡(Hardy-Weinberg equilibrium,HWE)。The simple sequence repeats （SSRs） were screened in the transcriptome from liver of Xenocypris argentea by high-throughput Illumina sequencing,and the polymorphism was analyzed as well. A total of 7272 SSRs were obtained. 47 out of 48 PCR primers,which were designed from tetranucleotide,pentanucleotide and hexanucleotide repeats units,were able to amplify steady and clear sequence bands by PCR. Polymorphism analysis of randomly selected 26 loci from above-mentioned 47 loci in Dongting Lake population showed that 21 of the 26 SSRs were proved to be polymorphic. The number of alleles per locus ranged from 3 to15,with an average of 7.29±3.94. The average values of observed heterozygosity （Ho）,expected heterozygosity （He）,polymorphism information content （PIC） and Shannon-Wiener index （H） were 0.5327±0.2114,0.6136±0.1964,0.5646±0.1980 and 1.3020±0.5779,respectively. The χ^2 test of Hardy-Weinberg equilibrium （HWE） revealed that the genetic disequilibrium was observed in 6 SSR loci from the 21 analyzed loci.

关 键 词：银鲴(Xenocypris argentea) 转录组测序 微卫星标记 多态性 

分 类 号：S917.4[农业科学—水产科学]