石河子垦区集约化奶牛场子宫炎主要病原菌的分离鉴定  

Isolation and Identification on Pathogenic Microorganism from Endometritis in Dairy Cows in Shihezi

作　　者：刘长彬[1] 卢春霞 罗小玲 侯扶琴 姜志涛 陈红莉[3] LIU Chang-bin;LU Chun-xia;LUO Xiao-ling;HOU Fu-qin;JIANG Zhi-tao;CHEN Hong-li(Institute of Animal Husbandry and Veterinary Science,Xinjiang Academy of Agriculture and Reclamation Sciences,Xinjiang Shihezi 832000,China;3.Analysis and Testing Center,Xinjiang Academy of Agriculture and Reclamation Sciences,Xinjiang Shihezi 832000,Chi-na;2.Xinjiang Western of Animal Husbandry Co.,LTD,Xinjiang Shihezi 832000,China)

机构地区：[1]新疆农垦科学院畜牧兽医研究所,新疆石河子832000 [2]新疆西部牧业股份有限公司,新疆石河子832002 [3]新疆农垦科学院分析测试中心,新疆石河子832000

出　　处：《西南农业学报》2018年第6期1300-1303,共4页Southwest China Journal of Agricultural Sciences

基　　金：国家科技支撑计划项目(2012BAD43B03);石河子科技计划课题(2014NY04)

摘　　要：【目的】为了解石河子垦区奶牛子宫炎主要致病菌感染情况,本研究采集石河子垦区4个集约化奶牛场的103头患有子宫炎的奶牛,分离病原菌及鉴定。【方法】利用子宫内取样器(专利号:ZL201420335941.6)采集患病奶牛子宫内粘膜或粘液样本206份,通过显微镜检查、细菌培养及分离、生化鉴定等方法进行病原学研究。【结果】结果显示,分离出8种病原菌共408株,其中葡萄球菌198株(占48.53%)、链球菌109株(占25.49%)、大肠杆菌85株(占20.83%)。从感染数据看,石河子垦区奶牛子宫炎以混合感染为主。【结论】石河子垦区集约化奶牛场子宫炎主要病原菌是葡萄球菌、链球菌和大肠杆菌。通过对该地区的奶牛子宫炎内致病菌的分离鉴定,为石河子垦区奶牛子宫炎的预防和治疗提供参考。[ Objective] To get the knowledge of the main cause of pathogenic bacteria of endometritis diseased dairy cows in Shihezi areas, this research randomly selected 103 endometritis diseased cows from four dairy cow fames, then separated and identified the pathogenic bacte- ria. [ Method ] Using intrauterine sampler （ Patent number ： ZL201420335941.6 ） 206 intrauterine mucous membrane or mucus samples of dis- eased cows were collected and detected by microscopic examination, bacteria cultivation, biochemical identification methods. [ Result] 408 trains for 8 kinds of pathogenic bacteria were detected out, including 198 trains of Staphylococcus aureus （48.53 % ） , 109 trains of Streptococ- cus （25.49 % ） ,85 trains of E. coli（20.83 % ）. In the 408 strains, from the data analysis, the main cause of the endometritis diseased cows is the mixed inflection of the pathogenic bacteria in Shihezi area. [ Conclusion] It suggested that the main pathogenic bacteria for cow endo- metritis in these four farms were Staphylococcus aureus, Streptococcus, E. coli. Based on Isolation and identification on pathogenic microor- ganism from endometritis of dairy cow in Shihezi area, it offers a reference metritis treatment on endometritis diseased cows of the intensive dairy farms in Shihezi areas.

关 键 词：石河子垦区 集约化奶牛场 奶牛 子宫炎 分离鉴定 

分 类 号：S823[农业科学—畜牧学]