岩藻黄醇标准样品的研制  

Development of fucoxanthinol certified reference material

作　　者：张怡评[1] 阎光宇 陈晖[1] 晋文慧[1] 方华[1] 陈伟珠[1] 洪专[1] 张文清[3] ZHANG Yiping1, YAN Guangyu2, CHEN Hui1, JIN Wenhui1, FANG Hua1, CHEN Weizhu1, HONG Zhuan1, ZHANG Wenqing3(1. Third Institute of Oceanography, State Oceanic Administration, Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen 361005, China; 2. Xiamen Ocean Vocational College, Xiamen361100, China;3. Fujian Provincial Governmental Hospital, Fuzhou 350003, China)

机构地区：[1]国家海洋局第三海洋研究所,福建省海洋生物资源开发利用协同创新中心,福建厦门361005 [2]厦门海洋职业技术学院,福建厦门361100 [3]福建卫生职业技术学院附属福建省级机关医院,福州350003

出　　处：《化学分析计量》2018年第4期6-10,共5页Chemical Analysis And Meterage

基　　金：海洋三所基本科研业务费(海三科2017007);厦门海洋研究开发院共建项目(K170102);海洋公益性行业科研专项(201405017);厦门海洋高技术产业基地创业创新共享服务平台项目(16PFW008SF15)

摘　　要：根据GB/T 15000.3–2008研制岩藻黄醇标准样品。以岩藻黄质提取物为原料,通过酶解、提取、柱层析、制备分离获得高纯岩藻黄醇单体,采用红外光谱、高分辨质谱和核磁共振波谱等方法进行结构确证。将样品分装为150瓶,随机抽取15个样品,采用高效液相色谱–紫外检测法进行稳定性、均匀性检验。检验结果表明,在95%的置信区间范围内本样品均匀性良好,于–18℃条件下保存12个月,其稳定性良好。经8家具有分析资质的实验室进行协同定值,岩藻黄醇标准样品特性标准值(纯度)为99.35%,相对扩展不确定度为0.49%(k=1.96)。研制的岩藻黄醇标准样品可用于岩藻黄醇相关产品的检验和质量控制。Fucoxanthinol reference material was developed according to GB/T 15000.3–2008. Fucoxanthinol was made from the Fucoxanthin. High purity fucoxanthinol monomer was obtained from the extracting of fucoxanthin by enzymolysis, extraction, column chromatography and separation. The structure of fucoxanthinol certified reference material was confirmed by infrared spectroscopy,high resolution mass spectral and nuclear magnetic resonance method. Fucoxanthinol samples was divided into 150 bottles, and 15 bottles of samples were randomly taken. The homogeneity and stability testing were carried out by high performance liquid chromatography–ultraviolet detector. The detection results indicated that homogeneity and stability of the fucoxanthinol reference material were well, the period of fucoxanthinol storage was 12 months at –18℃ . A cooperative certification was conducted by 8 laboratories with analytical qualifications. The certified value（purity） of the reference material of fucoxanthinol was 99.35% with relative expaned uncertainty of 0.49%（k=1.96）. The developed reference material of fucoxanthinol can be used for detection and quality control of fucoxanthinol related products.

关 键 词：岩藻黄醇 标准样品 均匀性 稳定性 定值 不确定度 

分 类 号：O656[理学—分析化学]