Ran对黑腹果蝇Kc细胞耐受DDT的影响  

Analysis of Ran and DDT Resistance in Drosophila Melanogaster Kc Cells

作　　者：柏骞 陈露[1] 常春洁 刘雅慧 程罗根[1] Bo Qian;Chen Lu;Chang Chunjie;Liu Yahui;Cheng Luogen(School of Life Sciences,Nanjing Normal University,Nanjing 210023,China)

机构地区：[1]南京师范大学生命科学学院,江苏南京210023

出　　处：《南京师大学报（自然科学版）》2018年第2期83-88,共6页Journal of Nanjing Normal University(Natural Science Edition)

基　　金：国家自然科学基金(31071974;31460482)

摘　　要：通过DDT刺激黑腹果蝇Kc细胞,着重讨论基因Ran的表达量变化情况.应用实时荧光定量PCR,检测不同浓度DDT诱导刺激条件下,Kc细胞内基因Ran表达量变化.利用流式细胞仪,检测不同浓度DDT诱导下细胞的凋亡率.结果表明,20 mg/L DDT处理的细胞,Ran表达水平最高.同时,在该浓度下,细胞凋亡率明显减弱,其变化趋势与Ran表达变化趋势基本一致.DDT可以显著提高Kc细胞Ran基因表达,降低细胞凋亡率.为了进一步验证Ran的功能,进行了Ran的过表达和干扰,并检测细胞凋亡情况.结果显示Ran的干扰能够明显提高细胞凋亡率.实验表明,Ran对细胞具有一定保护作用,Ran有可能是一个与杀虫剂耐受性相关的基因之一,并为进一步分析害虫应激反应及分子毒理提供研究依据.In this paper, the Kc cells of Drosophila melanogaster were stimulated by Dichlorodiphenyltrichloroethane （DDT）,we focus on the changes in the expression of Ran.Real-time fluorescence quantitative PCR was used to detect the changes in gene expression of Ran in Kc cells under different concentrations of DDT induced stimulation. Flow cytometry was used to detect the apoptosis rate of cells with different concentrations of DDT.The results showed that the expression of Ran in the cells treated by 20 mg/L DDT was the highest.At the same time,the apoptosis rate decreased obviously at this concentration,the trend of cell apoptosis was basically consistent with the change of Ran expression.DDT can significantly increase the expression of Ran gene in Kc cells and reduce the rate of apoptosis. In order to further verify the function of Ran,we carried out the overexpression and interference of Ran.The cell apoptosis was detected.The results showed that the interference of Ran could significantly increase the rate of apoptosis.The experiment showed that Ran had a certain protec-tive effect on the cells.Ran may be one of the genes associated with insecticide tolerance,which provides a basis for further analysis of the stress response and molecular toxicology of insect pests.

关 键 词：RAN DDT 应激 耐受性 细胞凋亡 

分 类 号：Q965.9[生物学—昆虫学]