失眠动物模型HPA轴和表观遗传修饰的变化及交泰丸的干预作用  

Changes of HPA Axis and Epigenetic Modification in Insomnia Animal Models and the Intervening Effect of Jiaotai Pill

作　　者：赵俊云[1] 杨晓敏[1] 胡秀华[1] 曹 简郭血骄 杨向竹[1] 郭健[1] ZHAO Junyun;YANG Xiaomin;HU Xiuhua;CAO Yan;JIAN GUO Xuejiao;YANG Xiangzhu;GUO Jian(Beijing Unisersity of Chinese Medicine,Beijing 100029,China)

机构地区：[1]北京中医药大学,北京100029

出　　处：《中医药学报》2018年第4期19-21,共3页Acta Chinese Medicine and Pharmacology

基　　金：中央高校基本科研业务费专项资金资助(2017JYBJS151)

摘　　要：目的:探讨交泰丸对PCPA致失眠大鼠血清HPA轴和表观遗传修饰的影响。方法:腹腔注射PCPA复制大鼠失眠模型,灌胃交泰丸干预3 d,ELISA法检测外周血血清HPA轴激素ACTH、CORT、CRF水平及表观遗传修饰主要酶DNMT1、HDAC的活性和血清Acetyl-Histone H3的水平。结果:PCPA致失眠大鼠的血清HPA轴激素水平上升,但与正常组无显著差异,交泰丸干预可显著降低失眠大鼠的血清HPA轴激素水平。失眠大鼠的血清DNMT1活性较正常组显著上升,交泰丸干预可显著降低失眠大鼠的血清DNMT1活性。PCPA致失眠大鼠的血清Acetyl-Histone H3水平上升,但与正常组无显著差异,交泰丸干预可显著降低失眠大鼠的血清Acetyl-Histone H3水平。结论:交泰丸可通过调节HPA轴激素水平治疗心肾不交型失眠。交泰丸可能通过DNA去甲基化以及组蛋白乙酰化等表观遗传修饰恢复机体的内稳态。Objective： To investigate the effect of Jiaotai Pill on serum HPA axis and epigenetic modification in PCPA-induced insomnia rats. Methods： The model of insomnia was induced by intraperitoneal injection of PCPA in rats. The rats were administrated with Jiaotai Pill（ Cinnamon： Berberine = 10： 1） for 3 days. Levels of ACTH,CORT,and CRF,enzyme activities of epigenetic modification,such as DNMT1 and HDAC,as well as serum level of Acetyl-Histone H3 were measured by means of ELISA mothod. Results： Serum hormones of HPA-axis were increased in PCPA-induced insomnia rats,but there was no significant difference between the model rats and the normal rats; Jiaotai Pill could significantly reduce the hormone levels. Activity of serum DNMT1 was significantly higher in the insomnia rats than that in the normal rats; which could be obviously reduce by the intervention of Jiaotai Pill. Serum levels of Acetyl-Histone H3 was increased in insomnia rats,however,there was no significant difference between the insomnia rats and the normal rats. Jiaotai Pill could significantly reduced the level of Acetyl-Histone H3. Conclusion： Jiaotai Pill can regulate the hormone levels of HPA-axis when treating insomnia of the pattern of abnormal physiological coordiantion between heart-yang and kidney-yin,which is achieved by restoring homeostasis and by epigenetic modifications,such as DNA demethylation and histone acetylation.

关 键 词：失眠 交泰丸 HPV轴 表观遗传修饰 

分 类 号：R285.5[医药卫生—中药学]