黄芩药材商品规格与内在质量相关性研究  

Correlation Research between the Product Specification and Intrinsic Quality of Scutellaria baicalensis Georgi.

作　　者：刘婷[1,2] 孔铭 刘丽芳[1] 李松林[2,3] LIU Ting;KONG Ming;LIU Lifang;LI Songlin(State Key Laboratory of Natural Medicines,China Pharmaceutical University,Nanjing 210009,China;Department of Metabolomics,Jiangsu Province Academy of TCM and Jiangsu Branch of China Academy of Chinese Medical Sciences,Nanfing 210028,China;Department of Pharmaceutical Analysis,Affiliated Hospital of Integrated Traditional Chinese and Western Medicine to Nanjing University of Chinese Medicine,Nanjing 210028,China)

机构地区：[1]中国药科大学天然药物活性组分与药效国家重点实验室,江苏南京210009 [2]江苏省中医药研究院和中国中医科学院江苏分院中药代谢组研究室,江苏南京210028 [3]南京中医药大学附属中西医结合医院中药质量研究室,江苏南京210028

出　　处：《中医药学报》2018年第4期41-47,共7页Acta Chinese Medicine and Pharmacology

摘　　要：目的:确定黄芩不同商品规格与主要化学成分之间的相关性。方法:采用高效液相色谱法,色谱柱为Altima^(TM)C18柱(4.6 mm×250 mm,5μm),流动相为乙腈-0.1%磷酸水(梯度洗脱),检测波长为280 nm,流速为1.0 m L/min,同时测定黄芩中黄芩苷、汉黄芩苷、黄芩素、汉黄芩素、白杨素、千层纸素A、夏佛塔苷、芹菜素和毛蕊花糖苷的含量。结果:来自不同产地(河北、山东、山西)一等及二等条芩药材各指标成分含量存在差异,其中,山东二等条芩药材中黄芩苷、汉黄芩苷含量最高,依次为19.39%、3.46%,河北一等条芩药材中黄芩苷、汉黄芩苷含量最低,依次为15.95%、2.71%,山西一等条芩药材中白杨素、千层纸素A含量最高,依次为0.14%、0.21%,山东一等及二等条芩药材中黄芩素、汉黄芩素含量最低,依次为0.44%、0.08%。相关性分析结果显示黄芩等级越高,黄芩苷、千层纸素A含量反而越低。聚类分析结果显示传统黄芩药材商品规格与内在整体质量不一致。结论:传统黄芩药材的商品规格以黄芩外观长度、粗细划分,与化学成分没有必然联系,无法体现黄芩内在质量,建议开展以内在质量和临床疗效为依据的黄芩药材分级研究,将药材外观性状、内部化学组分和疗效结合起来进行量化研究,完善黄芩药材商品等级规范。Objective： To determine the corrlelation between different specifications and the main chemical components of Scutellaria baicalensis Georgi.（ SBG）. Methods： HPLC method was adopted to simultaneously determine the contents of baicalin,wogonoside,baicalein,wogonin,chrysin,oroxylin-A,schaftoside,apigenin and acteoside. The analysis was performed on a Altima TMC18 column（ 4. 6 mm × 250 mm,5 μm） with a gradient mobile phase of acetonitrile-0. 1%phosphoric acid solution system at the flow rate of 1. 0 m L/min. The detective wavelength was set at 280 nm. Results：There were differences in the content of each index of first and second grade SBG from different sources（ Provinces of Hebei,Shandong and Shanxi）. The contents of baicalin（ 19. 39%） and wogonoside（ 3. 46%） in the second grade SBG from Shandong province were the highest,compared to those of the first grade SBG from Hebei province,which were15. 95% and 2. 71% respectively. In terms of the contents of chrysin and oroxylin-A,they were the highest in the first grade SBG from Shanxi province,which were 0. 14% and 0. 21%. In terms of the contents of baicalein and wogonin,they were the lowest in the first and second grade SBG from Shandong province,which were 0. 44% and 0. 08%. Correlation analysis showed that the higher the grade of SBG was,the lower the contents of baicalin and oroxylin-A would be. Cluster analysis showed that the traditional product specifications of SBG were inconsistent with the internal quality.Conclusion： Traditional product specifications of SBG were classified by the length and thickness,which has no certain relation with chemical components and can not reflect the inherent quality of SBG. It is recommended to carry out the classification of SBG on the basis of the intrinsic quality and clinical efficacy. To improve the quality standard of SBG the appearance,internal chemical components and curative effects on the quantitative research of SBG should be combined.

关 键 词：黄芩 商品等级 黄芩苷 汉黄芩苷 黄芩素 

分 类 号：R288[医药卫生—中药学] R284.1[医药卫生—中医学]