梅花鹿茸快速生长及骨化期相关差异表达蛋白分析  

Analysis of Differential Proteins of Sika Deer Antler at Rapid Growth and Ossification Stages

作　　者：幺宝金 张梅[2] 刘美辰 王群 刘宇昕 赵雨 YAO Bao-Jin;ZHANG Mei;LIU Mei-Chen;WANG Qun;LIU Yu-Xin;ZHAO Yu(Chinese Medicine and Bioengineering Research and Development Center,Changchun University of Chinese Medicine,Changchun 130117,China;Innovation Practice Center,Changchun University of Chinese Medicine,Changchun 130117,China)

机构地区：[1]中医药与生物工程研究开发中心创新实践中心,长春130117 [2]中医药与生物工程研究开发中心长春中医药大学,长春130117

出　　处：《分析化学》2018年第7期1152-1159,共11页Chinese Journal of Analytical Chemistry

基　　金：本文系吉林省教育厅“十三五冶科学技术研究项目(No.JJKH20170721KJ)和吉林省科技厅优秀青年人才基金项目(No.20170520044JH)资助

摘　　要：采用同位素标记相对定量(iTRAQ)技术、超高效液相色谱-质谱联用技术和生物信息学分析方法对东北梅花鹿快速生长期(60 d)与骨化期鹿茸(90 d)进行比较蛋白质组学研究,共鉴定出127种差异蛋白。与骨化期鹿茸比较,快速生长期鹿茸中显著上调的差异蛋白80种,显著下调差异蛋白47种,这些差异表达蛋白主要分布在细胞外基质、核小体、珠蛋白-血红蛋白复合物、肌动蛋白丝、内质网-高尔基中隔室、内质网官腔及核内膜等部位。快速生长期鹿茸显著上调的差异蛋白主要是参与血氧运输、神经生长和再生、软骨和骨组织发育及ATP合成的功能蛋白,而显著下调的差异蛋白主要为参与软骨内骨化过程的功能蛋白,这些蛋白在鹿茸不同生长时期表达量的变化与鹿茸的快速生长和骨化进程密切相关。本研究为系统揭示鹿茸快速生长及骨化的分子机制提供了基础数据,同时对研究鹿茸的药效物质基础及临床应用具有重要的指导意义。Differential proteomics analysis of Sika deer antlers at rapid growth stage （60 d） and ossificationstage （90 d） was performed by isobaric tags for relative and absolute quantitation （ iTRAQ）, ultra highperformance liquid chromatography and mass spectrometry technologies. A total of 127 differential proteinswere identified. Compared with the ossification stage, 80 differential proteins were significantly up-regulatedand 47 differential proteins were significantly down-regulated at the rapid growth stage. These differentialproteins were mainly distributed in the regions of extracellular matrix, nucleosome, haptoglobin-hemoglobincomplex, actin filament, endoplasmic reticulum-Golgi intermediate compartment, endoplasmic reticulumlumen, and endometrium, etc. The up-regulated differential proteins were mainly involved in the regulationsof oxygen transport in the blood, nerve growth and regeneration, cartilage and bone development and ATPsynthesis compared with ossification stage, and the down-regulated differential proteins were mainly involved inthe endochondral ossification process. The changes of protein expression at different growth stages were closelyrelated to antler rapid growth and ossification. Therefore, the results of this study provided a basic data fordiscovering the molecular mechanisms of antler rapid growth and ossification, and it was of great significancefor further study of the pharmacological basis and clinical application of antlers.

关 键 词：梅花鹿茸 快速生长期 骨化期 同位素标记相对定量技术 蛋白质组学 差异蛋白 

分 类 号：S825[农业科学—畜牧学]