人工培养和野生铅绿褶菇抗氧化活性研究  

Studies on Antioxidant Activity of Artificial Culture and Wild Chlorophyllum molybdites

作　　者：林群英[1] 龙良鲲 向准[3] 康超 邓春英[3] LIN Qun-ying;LONG Liang-kun;XIANG Zhun;KANG Chao;DENG Chun-ying(Nanjing Institute for the Comprehensive Utilization of Wild Plants,Nanjing,Jiangsu 210042,China;Nanjing Forestry University,College of Chemical Engineering,Department of Pharmaceutical Engineering,Nanjing,Jiangsu 210037,China;Guizhou Academy of Sciences,Guiyang,Guizhou 550001,China;Guizhou Institute of Biology,Guiyang,Guizhou 550000,China)

机构地区：[1]中华全国供销合作总社南京野生植物综合利用研究院,江苏南京210042 [2]南京林业大学化学工程学院制药工程系,江苏南京210037 [3]贵州科学院,贵州贵阳550001 [4]贵州省生物研究所,贵州贵阳550000

出　　处：《福建农业学报》2018年第5期534-537,共4页Fujian Journal of Agricultural Sciences

基　　金：贵州省科学技术基金(黔科合J字[2015]2057号);贵州省科技计划项目(黔科合支撑[2017]2513)

摘　　要：利用秀丽线虫模型及DPPH·清除试验对铅绿褶菇进行体内和体外抗氧化活性研究。结果显示,铅绿褶菇发酵液可明显提升秀丽线虫的抗氧化能力,0.5mg·mL-1时,可显著提高秀丽线虫的平均寿命,比空白对照组提高了70%,野生未开伞子实体的抗氧化能力较开伞子实体强;未开伞子实体水提液0.5~1.0mg·mL-1处理的秀丽线虫平均寿命分别为4.85d和5.06d,显著高于空白对照组。发酵液对DPPH·的清除率最高,达98.14%,其次是开伞子实体水提液92.86%,未开伞子实体水提液为85.71%。表明铅绿褶菇可成为抗氧化物开发的潜在生物材料。Lifespan of Caenorhabditis elegans in vivo,along with the DPPH radical scavenging test in vitro,was used to evaluate the anti-oxidative activity of Chlorophyllum molybdites.The results showed that the C.molybdites fermentation broth increased the resistance of the nematodes to oxidative stress.At 0.5 mg·mL-1,the broth extended the mean lifespan of the paraquat-treated C.elegans 70% longer as compared to that of CK.The antioxidative activity of the water extract of immature fruitbodies was greater than that of the mature fruitbodies of C.molybdites.When treated with the water extract of immature mushrooms at 0.5 and 1.0 mg·mL-1,the mean lifespans of the nematodes were 4.85 dand 5.06 d,respectively,which were significantly longer than that of CK.The DPPH scavenging rate of the fermentation broth was the highest,at 98.14%,followed by that of the water extract of mature fruitbodies,at 92.86%,and that of the water extract of immature fruitbodies,at 85.71%.The findings suggested that C.molybdites could potentially be a new source for producing antioxidant from a biomaterial.

关 键 词：铅绿褶菇 抗氧化 DPPH· 秀丽线虫 

分 类 号：Q939.9[生物学—微生物学]