冷应激对雏鹌鹑十二指肠肠道菌群的影响  

Effects of cold stress on the intestinal flora in quail duodenum

作　　者：刘春朋 付晶 曾依翎 郭思璇 曹楠 田允波 许丹宁 LIU Chunpeng;FU Jing;ZENG Yiling;GUO Sixuan;CAO Nan;TIAN Yunbo;XU Danning(Guangdong Province Key Laboratory of Waterfowl Healthy Breeding,Guangdong 510225,China;College of Animal Science ＆ Technology,Zhongkai University of Agricultural and Engineering,Guangdong 510225,China)

机构地区：[1]广东省水禽健康养殖重点实验室,广东广州510225 [2]仲恺农业工程学院动物科技学院,广东广州510225

出　　处：《仲恺农业工程学院学报》2018年第2期1-7,共7页Journal of Zhongkai University of Agriculture and Engineering

基　　金：广东省科技计划(2017B020202010);2017-2018年度中国-白俄罗斯政府间科技交流(CB01-13);广州市科技计划(201604020061);广东省现代产业技术体系(2016LM1113)资助项目

摘　　要：为探索促进动物冷适应的方法,使动物在寒冷环境中能够保持正常生理机能,在建立雏鹌鹑急、慢性冷应激模型的基础上,应用变性凝胶电泳技术(PCR-DGGE),检测急、慢性冷应激对十二指肠组织肠道菌群的影响.结果表明,急、慢性冷应激均可以导致雏鹌鹑十二指肠内肠道微生物数量和种类的改变.冷应激会引起雏鹌鹑肠道生物屏障功能受损.In order to explore the method of promoting animal cold adaptation,and enable animals can maintain normal physiology in cold environment,this study was built cold stress model of quails,using the PCR-DGGE method to detect the change of intestinal microflora in quail duodenum which induced by acute and chronic cold stress groups. The results showed that both acute and chronic cold stress could cause changes in the number and types of intestinal microbiota in the duodenum. It concluded that cold stress could lead to impaire intestinal biologic barrier function.

关 键 词：冷应激 鹌鹑 十二指肠 肠道菌群 

分 类 号：S814.1[农业科学—畜牧学]