人支气管上皮细胞16HBE和其经硫化镍、反式-BPDE诱导的恶性转化细胞对脂质体转染效率的差异性比较  

Comparison of the Transfection Efficiency through Liposome between 16HBE and the Malignant Transformation Cells Induced by Nickel Sulfide and Anti-Benzo(a) pyrene

作　　者：夏盛源 王远航 朱蔚云[1] 张岚[1] XIA Shengyuan;WANG Yuanhang;ZHU Weiyun;ZHANG Lan(GMU-GIBH Joint School of Life Sciences,Guangzhou Medical University,Guangzhou 511436,Guangdong,China;Jiaxing Center for Disease Control and Prevention,Jiaxing 314050,Zhejiang,China)

机构地区：[1]广州医科大学-中国科学院广州生物医药与健康研究所联合生命科学学院,广东广州511436 [2]嘉兴市疾病预防控制中心,浙江嘉兴314050

出　　处：《激光生物学报》2018年第2期148-154,共7页Acta Laser Biology Sinica

基　　金：国家自然科学基金项目(81102159);广东省自然科学基金项目(2016A030313567);广州市教育局广州市属高校"羊城学者"科研项目(12A010D);广州市科技计划项目(201607010035)

摘　　要：目的:通过脂质体法转染不同荧光蛋白质粒到多种接种密度细胞的效率差异探讨恶性转化细胞的癌变过程。方法:以16HBE、结晶型NiS诱导的16HBE恶性转化细胞和反式-BPDE诱导的16HBE恶性转化细胞为实验对象,采用不同的细胞接种密度,用脂质体法对它们分别转染两种不同的表达荧光蛋白的质粒,通过荧光显微镜观察细胞的转染情况。结果:无论转染eGFP质粒还是GFP-LC3质粒,在多种细胞密度下,正常细胞16HBE都比恶性转化细胞的转染效率低。结论:恶性转化细胞在癌变过程中可能发生了细胞膜通透性的改变。Objective： To investigate the carcinogenesis of malignant transformation cells by comparing the difference in transfection efficiency of exogenous plasmids through liposome between normal cells and malignant transfected cells.Methods： Two types of plasmids that express the fluorescent proteins were transfected with liposome into cells such as16 HBE and its malignant transformation cells induced by crystalline nickel sulfide and anti-benzo（ a） pyrene separately,and the transfection were observed by fluorescent microscope. Results：In a range of cell density,the transfection efficiency of normal cells 16 HBE is lower than that of the malignant transfected cells both with the plasmids e GFP and GFPLC3. Conclusion： Inconsistency in membrane permeability between normal cells and malignant transfected cells leads to inconsistency in transfection efficiency.

关 键 词：脂质体 转染效率 恶性转化细胞 EGFP GFP-LC3 

分 类 号：Q291[生物学—细胞生物学]