“一测多评”法测定青阳参中3个苯乙酮类化合物的含量  

Determination of three acetophenones in Cynanchum otophyllum by quantitative analysis of multi-components with a single-marker(QAMS)

作　　者：杨铭 李欣[1] 杨庆雄 YANG Ming;LI Xin;YANG Qingxiong(School of Chemistry and Materials Science,Guizhou Normal University,Guiyang,Guizhou 550001,China;School of Karst Science,Guizhou Normal University,Guiyang,Guizhou 550001,China;State Engineering Technology Institute for Karst Deserffieation Control,Guiyang,Guizhou 550001,China)

机构地区：[1]贵州师范大学化学与材料科学学院,贵州贵阳550001 [2]贵州师范大学喀斯特研究院,贵州贵阳550001 [3]国家喀斯特石漠化防治工程技术研究中心,贵州贵阳550001

出　　处：《贵州师范大学学报（自然科学版）》2018年第3期40-45,共6页Journal of Guizhou Normal University：Natural Sciences

基　　金：国家十三五重点研发计划项目(No.2016YFC0502600);国家自然科学基金项目(No.31760091)

摘　　要：采用"一测多评"法快速测定青阳参药材中对羟基苯乙酮(A),2,5-二羟基苯乙酮(B),2,4-二羟基苯乙酮(C)的含量。以青阳参中3个苯乙酮类成分为指标,通过采用UPLC方法测得校正因子fA/fB=2.716,fA/fC=1.289,实现一测多评,且校正因子精密度和重复性良好。建立的方法可用于青阳参药材中苯乙酮类成分的质量评价,结果稳定方法可靠。A method of quantitative analysis of multi-components with a single-marker（QAMS） was used to rapidly determine the contents of three kinds of acetophenone,including p-hydroxyacetophenone（A）,2,5-dihydroxyacetophenone（B） and 2,4-dihydroxyacetophenone（C）. In addition,the practicability and applicability of the method were also examined. Taking acetophenone in C. otophyllum as the index component,a method of UPLC was used to measure and ensure the relative correlation factors（f） between A and B（denoted as fA/fB）,A and C（denoted as fA/fC）,achieving the objective of QAMS,i. e. ： fA/fBand fA/fCwere 2. 716 and 1. 289,respectively. Results revealed that both the two factors were good in reproducibility and precision. QAMS was a stable and reliable method.Thus,it could be used to evaluate the quality of acetophenone in C. otophyllum.

关 键 词：一测多评 相对校正因子 青阳参 苯乙酮 

分 类 号：O657.7[理学—分析化学] TQ041[理学—化学]