烟曲霉对肺微血管内皮细胞通透性的影响及可能机制  

Effect of Aspergillus fumigatus on pulmonary microvascular endothelial cell permeability and its mechanism

作　　者：宋珺[1] 韩菁[1] 孙越[1] 施伟民[1] SONG Jun;HAN Jing;SUN Yue;SHI Wei-min(Dept.of Dermatology,First People＇s Hospital,Shanghai Jiao Tong University,Shanghai 200080,China)

机构地区：[1]上海交通大学附属第一人民医院皮肤科,上海200080

出　　处：《同济大学学报（医学版）》2018年第3期19-23,共5页Journal of Tongji University(Medical Science)

基　　金：国家重点基础研究发展计划(973计划)(2013CB531604);上海交通大学附属第一人民医院院级项目(11B06)

摘　　要：目的探讨烟曲霉对肺微血管内皮细胞通透性的影响及其机制。方法利用激光共聚焦扫描显微镜检测人肺微血管内皮细胞肌动蛋白(F-actin)和细胞跨膜电阻仪测定细胞跨膜电位,以观察细胞通透性的改变,并利用p38 MAPK抑制剂、ROCK抑制剂和蛋白激酶C抑制剂探讨其机制。结果烟曲霉处理后肺微血管内皮细胞Factin染色的荧光强度显著下降(P<0.01),烟曲霉处理组也出现细胞跨膜电阻值的显著下降(P<0.01)。与单独烟曲霉处理组比较,SB203580(20μmol/L,p38 MAPK抑制剂)干预组、Y27632(20μmol/L,ROCK抑制剂)干预组以及LY317615(10μmol/L,蛋白激酶C抑制剂)干预组的跨膜电阻值均显著上升(P<0.05)。结论烟曲霉处理致肺微血管内皮细胞通透性增加,其机制可能涉及p38 MAPK、ROCK激酶以及蛋白激酶C通路。Objective To investigate the effect of Aspergillus fumigatus（ AF） on human pulmonary microvascular endothelial cell（ HPMVEC） permeability and the related mechanisms.Methods HPMVECs were treated with AF, cell F-actin was observed with confocal laser scanning microscopy and cell transmembrane resistance was measured. In addition, the cell permeability changes in AF-treated HPMVECs were also examined with intervention of p38 MAPK inhibitor SB203580（ 20 μmol/L）,ROCK inhibitor Y27632（ 20 μmol/L）,or PKC inhibitor LY317615（ 10 μmol/L）,respectively. Results AF treatment induced a significant reduction of Factin fluorescence intensity（ P〈0.01） and a significant reduction of cell transmembrane resistance（ P〈0.01） in HPMVECs. Furthermore,treatment with SB203580,Y27632 or LY317615 significantly inhibited（ P〈0.05） AF-induced increase of cell transmbrane resistance in HPMVECs. Conclusion AF can increase cell permeability in HMVECs,in which the MAPK（ p38）,ROCK and PKC pathways may be involved.

关 键 词：烟曲霉 肺微血管内皮细胞 细胞通透性 

分 类 号：R751[医药卫生—皮肤病学与性病学]