通络醒脑泡腾片对VD模型大鼠视交叉上核生物钟基因表达的影响  

Effect of Tongluo Xingnao Effervescent Tablets on the expression of the nuclear clock gene in the suprachiasmatic nucleus of VD model rats

作　　者：潘婷 魏江平[1,2] 徐世军 PAN Ting;WEI Jiangping;XU Shijun(School of Medicine,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;Institute of Material Medica Integration and Transformation for Brain Disorders,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China)

机构地区：[1]成都中医药大学药学院,四川成都611137 [2]成都中医药大学中医脑病药物整合转化研究所,四川成都611137

出　　处：《药学研究》2018年第6期311-314,共4页Journal of Pharmaceutical Research

基　　金：国家自然科学基金重点项目(No.81430100)

摘　　要：目的考察通络醒脑泡腾片对血管性痴呆(VD)模型大鼠视交叉上核生物钟基因表达的影响。方法采用双侧结扎颈总动脉法(2-VO法)制备血管性痴呆大鼠模型,采用Morris水迷宫评价其认知功能;反转录聚合酶链式反应(RT-PCR)法检测各组大鼠视交叉上核生物钟基因Clock、Bmal、Per2基因的表达。结果通络醒脑泡腾片能够改善血管性痴呆模型大鼠学习记忆功能,可上调模型大鼠视交叉上核Bmal、Clock和Per2基因表达(P>0.05)。结论通络醒脑泡腾片调节血管性痴呆模型大鼠认知能力与其调节视交叉上核生物钟基因Bmal、Clock和Per1有关。Objective To explore the effect of Tongluo Xingnao Effervescent Tablets on the expression of Clock,Bmal and Per2 genes in the suprachiasmatic nucleus of VD model rats.Methods VD rat model was prepared by bilateral common carotid arteries ligation（ 2-VO） method.Morris water maze was used to evaluate the learning and remembering ability.The RT-PCR was used to analyse the mRNA expression of Clock,Bmal and Per2 genes on the suprachiasmatic nucleus.Results Tongluo Xingnao Effervescent Tablets can improve learning and memory function of VD rats,and upregulate the expression of Bmal,Clock and Per2 genes in suprachiasmatic nucleus of rats（ P〉0.05）.Conclusion Tongluo Xingnao Effervescent Tablets can improve the cognitive function of vascular dementia rats,which was relate to the regulation of Bmal,clock and Per2 genes on the suprachiasmatic nucleus.

关 键 词：通络醒脑泡腾片 血管性痴呆 认知能力 生物钟基因 

分 类 号：R965[医药卫生—药理学]