Ta_2O_5纳米管结合BMP2指节肽(BKP)对种植体骨整合的影响  

Effects of Ta_2O_5 nanotubes functionalized with BMP2 knuckle peptide(BKP) on osseointegration of implants

作　　者：马苑萍 张振庭[2] 张欣泽 王昊[1] MA Yuan-ping;ZHANG Zhen-ting;ZHANG Xin-ze;WANG Hao(Department of Stomatology,Beijing Tian Tan Hospital,Capital Medical University,Beijing 100050 China)

机构地区：[1]首都医科大学附属北京天坛医院口腔科,医师北京100050 [2]首都医科大学附属北京口腔医院,主任医师教授北京100050

出　　处：《口腔颌面修复学杂志》2018年第4期241-245,共5页Chinese Journal of Prosthodontics

基　　金：国家自然科学基金(项目编号:81470759)

摘　　要：目的:通过体内实验揭示BMP2指节肽(BKP)结合后的Ta_2O_5纳米管是否具有促进种植体表面骨整合的能力。方法:以多巴胺为中间介质,将BKP结合于Ta_2O_5纳米管表面,通过接触角测量和X射线光电子能谱检测BKP和Ta_2O_5纳米管的成功结合。将种植体植入新西兰白兔的胫骨内,采用实时定量PCR检测术后1、2、3、4周种植体表面成骨相关基因ALP和Col-I的表达,制作骨-种植体不脱钙磨片,观察术后2、4、8周种植体周围骨的组织学表现。结果:BKP成功结合至Ta2O5纳米管表面。相对于两组对照组,实验组表面基因表达量有显著的提高且在骨-种植体接触面积和新骨形成方面有明显增加(P<0.05)。结论:在Ta_2O_5纳米管表面附着BKP可明显促进种植体骨整合,这种表面处理方法可用于改善钽种植体的临床应用。Objective： To explore the effects of BMP2 knuckle peptide（BKP）, in combination with Ta2O5 nanotubes, on implant osseointegration in vivo. Methods： Polydopamine was immobilized onto Ta2O5 nanotubes as an intermediate layer,and then BKP was conjugated onto the nanotubes. The successful fabrication of BKP was conιrmed by contact angle measurement and X-ray photoelectron spectroscopy. The implants were then inserted into the tibiae of rabbits, the gene expression of alkaline phosphatase and collagen-I was examined by real-time PCR at 1, 2, 3 and 4 weeks after implantation, and the histological features around the implants on non-decalciιed sections were investigated at 2, 4, and 8 weeks after implantation. Results： There was a signiιcant increase in gene expression levels and bone-implant contact of the bone attached to implants with immobilized BKP compared with the other two control groups. Conclusion： The surface functionalization of Ta2O5 nanotubes with BKP was beneιcial for osseointegration and this approach could be further developed to improve tantalum-based implants for various applications.

关 键 词：BMP2指节肽(BKP) Ta2O5纳米管 多巴胺 基因表达 骨整合 

分 类 号：R780.2[医药卫生—口腔医学]