用于鉴定小麦缺体-四体真实性的SSR分子标记的筛选  

Screening of SSR Molecular Markers for Identifying the Authenticity of Nulli-Tetrasomes of Wheat

作　　者：卫笑 杨靖[1] 孙海燕[1] 曹银萍[1] 巴爱丽 李友勇[1] Wei Xiao;Yang Jing;Sun Haiyan;Cao Yinping;Ba Aili;Li Youyong(Henan Institute of Science and Technology/Collaborative Innovation Center of Modern Biological Breeding,Xinxiang Henan 45300)

机构地区：[1]河南科技学院/现代生物育种河南省协同创新中心,河南新乡453003

出　　处：《中国农学通报》2018年第20期47-54,共8页Chinese Agricultural Science Bulletin

基　　金：河南省基础与前沿计划重点项目"小麦BNS雄性不育系温敏不育基因染色体定位和差异表达分析"(122300410011);"小麦雄性不育BNS恢复基因连锁标记筛选和QTL定位"(162300410136)

摘　　要：旨在筛选一组数量较大,可靠性较高的SSR分子标记,用于辅助鉴定小麦缺体-四体的真实性。在小麦SSR分子标记图谱上,选取单一位点标记引物,然后在中国春和已鉴定的中国春缺体-四体DNA中扩增检测,筛选出带型清晰,易识别的单扩增产物标记。结果表明:在小麦21个连锁群上共选择标记150个,长短臂均有分布,最终筛选出标记67个,这些标记在中国春和所有非连锁的缺体-四体中扩增为阳性,在对应连锁的缺体-四体中扩增为阴性,扩增产物为单条带。该套引物均匀分布在小麦基因组染色体上,每个连锁群上最少3个,最多5个,扩增条带单一,且清晰易识别,能够有效地适用于所有中国春小麦缺体-四体及其他非整倍体的真实性鉴定。The aim is to screen a group of SSR（simple sequence repeat） molecular markers with relatively highquantity and reliability which could identify the authenticity of nulli-tetrasomes lines of wheat. The singlelocus makers on the SSR molecular marker map of wheat were selected, and then the makers were amplified inDNA of Chinese Spring and Chinese Spring nulli-tetrasomes lines. And then the primers that produced clearand easy-identified band were screened out. The results showed that a total of 150 SSR markers on 21 linkagemaps of wheat covering the long and short arms of each chromosome were selected. At last, 67 markers wereselected which showed positive amplification in Chinese Spring and non-linkage nulli-tetrasomes, andnegative amplification in the corresponding linkage nulli-tetrasomes. The production was the single band. Thisset of primers distributed evenly over all chromosomes of wheat genome, at least 3, up to 5 on eachchromosome, and their bands were single and easily identified, and could be efficiently used for identifying theauthenticity of Chinese Spring nulli-tetrasomes lines and other aneuploidy of wheat.

关 键 词：小麦 SSR分子标记 中国春 缺体-四体 真实性鉴定 

分 类 号：S512.101[农业科学—作物学] Q943.2[生物学—植物学]