临床分离CRKP生物膜形成与分子分型及药敏特点  

Biofilm Formation, Molecular Typing and Drug Susceptibility Characteristics of Carbapenem-Resistant Klebsiella Pneumoniae

作　　者：涂浩鹏 陈俊文 张波 胥志超 钟婉莹 陈宪娣 陈重 马孝煜 白冰 邓向斌 潘伟光 徐广健 余治健 邓启文 李多云 TU Hao-peng;CHEN Jun-wen;ZHANG Bo;XU Zhi-chao;ZHONG Wan-ying;CHEN Xian-di;CHEN Chong;MA Xiao-yu;BAI Bing;DENG Xiang-bin;PAN Wei-guang;XU Guang-jian;YU Zhi-jian;DENG Qi-wen;Li duo-yun(Nanshan Hospital Affiliated to Shenzhen University,Guangdong Shenzhen 518052)

机构地区：[1]深圳大学附属南山医院,广东深圳518052

出　　处：《深圳中西医结合杂志》2018年第13期1-4,共4页Shenzhen Journal of Integrated Traditional Chinese and Western Medicine

基　　金：国家自然科学基金资助课题(81601797);深圳市南山区级项目资助课题(2016010;2017013;2017015;2017026;2017027);深圳市三名工程经费资助课题

摘　　要：目的:探讨临床分离耐碳青霉烯肺炎克雷伯菌(CRKP)生物膜形成特点和多位点序列分型(MLST)及药敏的关系。方法:收集深圳大学附属南山医院2011–2016年分离出的CRKP,美国BD公司Phoenix TM100全自动细菌药敏鉴定仪检测常规药敏,采用聚合酶链式反应(PCR)方法分析其MLST分型,使用96孔结晶紫染色法进行生物膜光密度A(OD)值检测。结果:36株菌中检出24株生物膜阳性,12株生物膜阴性,其中19株弱阳性,5株中阳性,未发现强阳性菌株。MLST分布不太集中,MLST数量由高到低为37、11、15、273,没有序列分型(NT)数量最多,其中ST37和273都集中于生物膜弱阳性。生物膜阳性阿米卡星、氨曲南、氨苄西林、环丙沙星、头孢他啶、头孢噻肟和左氧氟沙星耐药率(含中介药敏)为8.3%、58.3%、95.8%、45.8%、58.3%、75.0%和50.0%。结论:碳青霉烯肺炎克雷伯菌生物膜形成能力比较强,在MLST中分布不集中。Objective To explore the relationship between the biofilm formation characteristics and the multilocus sequence typing（MLST） and susceptibility of Klebsiella pneumoniae. Methods Klebsiella pneumoniae was collected from 2011-2016 in our hospital.The phenotypes of conventional phytotoxicity were detected by BD company＇s Phoenix TM 100 automatic bacterial susceptibility tester.The MLST typing was analyzed by PCR and 96-well crystal violet staining was used. Results Among the 36 strains, 24 biofilms were detected positive, and 12 biofilms were negative, 19 of which were weakly positive, 5 were positive, and no strong positive strain was found. The distribution of MLST is not concentrated. The number of MLST is 37, 11, 15, and 273. The Chinese name is the largest number of No type（NT）, and ST37 and 273 are concentrated in the biofilm weak positive. The biofilm-positive amikacin, aztreonam, ampicillin, ciprofloxacin, ceftazidime, cefotaxime and levofloxacin resistance rates（including intermediate drug sensitivity） were 8.3%, 58.3%, 95.8 %, 45.8 %, 58.3 %, 75.0%, and 50.0%. Conclusion The biofi lm formation ability of carbapenem Klebsiella pneumoniae is relatively strong and its distribution is not concentrated in MLST.

关 键 词：耐碳青霉烯 肺炎克雷伯菌 生物膜 多位点序列分型 结晶紫染色法 

分 类 号：R446.5[医药卫生—诊断学]