非繁殖季节发情与乏情哈萨克羊下丘脑差异表达基因分析  

Differentially expressed genes in hypothalamus of Kazakh sheep estrus and anestrus in non-breeding season

作　　者：梁慧慧[1] 翟曼君[1] 梁艳萍 解一凡 曹少奇 赵宗胜[1] Liang Huihui;Zhai Manjun;Liang Yanping;Xie Yifan;Cao Shaoqi;Zhao Zongsheng(College of Animal Science and Technology,Shihezi University,Shihezi,Xinjiang 832003,China)

机构地区：[1]石河子大学动物科技学院

出　　处：《石河子大学学报（自然科学版）》2018年第2期145-152,共8页Journal of Shihezi University(Natural Science)

基　　金：国家自然科学基金项目(31260537)

摘　　要：通过构建数字基因表达谱(Digital Gene Expression,DGE)文库,筛选出非繁殖季节乏情与营养诱导的发情哈萨克羊下丘脑差异表达基因,为丰富绵羊发情机制奠定基础。本研究选取36只哈萨克羊随机分为补饲组和对照组各18只。非繁殖季节采集3只乏情和3只补饲后发情的哈萨克羊下丘脑,构建DGE文库并进行差异基因数字表达谱分析。结果表明:通过补饲能够使处于非繁殖季节的哈萨克羊发情,其发情率为44%,而对照组没有发情现象。经差异基因数字表达谱分析,补饲组发情与对照组乏情下丘脑共筛选出828个差异表达基因,其中728个上调,100个下调。GO功能富集分析表明,差异表达基因主要在神经元胞体、轴突、细胞膜上表达,具有调节GTP酶的活性等功能,这些基因还参与细胞通讯、信号调控、神经冲动传导、细胞定位等生物过程。通过Pathway分析发现,差异表达基因显著富集在Gn RH信号通路、神经营养因子的信号转导通路、胰岛素信号通路等31条信号通路。结合DGE文库及已有文献筛选出GNAQ、ERα、PI3K、POMC可能是参与营养诱导的调控哈萨克羊非繁殖季节发情相关通路的关键基因。In order to explore the mechanism of the sheep estrus, here, the differentially expressed genes in hypothalamus of Kazakh sheep estrus and anestrus was screened by constructing Digital Gene Expression（DGE） libraries in non-breeding season. 36 Kazakh sheep were randomly divided into experimental group（supplementary feeding） and control group, 18 cases in each group. The hypothalamus were collected from three Kazakh sheep of experimental group, which occur onset of estrus after supplementary feeding, and the three control group in non-breeding season to construct the DGE library. The differentially expressed genes were analyzed by digital expression profiling,the results showed that the rate of estrus was 44% in experimental group sheep in non-breeding season; while the control group have not yet occurred the estrus. The date showed that there were 828 differentially expressed genes between the experimental group and the control group, 728 of them up-regulated and 100 down-regulated. The GO enrichment analysis indicated that differentially expressed genes, which could regulate the GTPase activity or had other functions, were expressed mainly in the neuronal soma, axon, and cell membrane.Those genes also involved in the regulation of cell communication, cell signal transductions, nerve impulse conduction, cellular localization and other biological processes. The signaling pathway analysis revealed that differentially expressed genes were enriched significantly in Gn RH signaling pathway, neurotrophic factor（NTFs） related signaling pathway, insulin-signaling pathway and other signaling pathways, in total 31 signal pathway. Based on previous studies and our results, it is GNAQ, ERα, PI3 K and POMC gene that may be critical for Kazakh sheep to regulate estrus related pathways that involve nutrition induce with supplementary feeding in non-breeding season.

关 键 词：非繁殖季节 发情 乏情 DGE 下丘脑 差异表达基因 

分 类 号：S826.84[农业科学—畜牧学]