乌裕尔河流域富裕段麦穗鱼感染的吸虫囊蚴分子生物学调查  

Survey on metacercariae infection in Pseudorasbora parva by molecular biology method in Fuyu region of Wuyuer river basin

作　　者：高俊峰 张莹[2] 王丽坤 崔宇超[2] 侯美如[2] 路义鑫[1] GAO Jun-feng;ZHANG Ying;WANG Li-kun;CUI Yu-chao;HOU Mei-ru;LU Yi-xin(Northeastern Science Inspection Station,China Ministry of Agriculture Key Laboratory of Animal Pathogen Biology,Northeast Agricultural University,Harbin 150030,China;Department of Parasitology,Heilongjiang Institute of Veterinary Science,Qiqihar 161000,China)

机构地区：[1]东北农业大学农业部动物疫病病原生物学重点实验室/东北科学观测实验站,黑龙江哈尔滨150030 [2]黑龙江省兽医科学研究所寄生虫研究室,黑龙江齐齐哈尔161000

出　　处：《中国预防兽医学报》2018年第7期646-649,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：农业部动物疫病病原生物学东北科学观测实验站重点实验室开放课题农业部动物疫病病原生物学重点实验室东北科学观测实验站开放课题(DY201707)

摘　　要：为了解乌裕尔河流域富裕段麦穗鱼感染吸虫囊蚴的种类,本研究采集乌裕尔河流域富裕段麦穗鱼,利用人工消化法收集的不同形态的吸虫囊蚴,在显微镜下观察,根据囊蚴的形态特点进行分类。同时分别提取各种囊蚴的总DNA,利用PCR方法扩增其第二内转录间隔区(ITS2)基因序列并进行测序,将获得的基因序列与Gen Bank数据库相应的基因序列进行比对分析,构建系统发生树。结果显示乌裕尔河流域富裕段麦穗鱼主要感染3种吸虫囊蚴,分别为华支睾吸虫、东方次睾吸虫和日本全冠吸虫。本研究进一步证明形态学结合分子生物方法是鉴定淡水鱼中吸虫囊蚴感染种类的有效手段。In order to investigate the kinds of the trematode metacercariae infected Pseudorasbora parva in Fuyu region of Wuyuer river basin, P.parva were collected in this region and the trematode metacercariae were isolated using artificial digestion method. The trematode metacercariae were classified by morphological characteristics utilizing the microscope. The total DNA of each kind of metacercariae was extracted, and the second internal transcriptional space （ITS2） sequences were amplified by PCR and sequenced. Sequences were assembled and aligned with the sequences available in GenBank database, and the phylogenetic relationships were reconstructed based on ITS2 sequences with MP methods. The results showed that three kinds of metacercariae were identified in P.parva, named Clonorchis sinensis, Metorchis orientalis and Holostephanus dubinini, respectively. It is suggested that morphology combined with molecular biological methods is an effective method for identification of metacercariae in freshwater fish.

关 键 词：乌裕尔河流域 麦穗鱼 吸虫囊蚴 形态学 分子生物学 

分 类 号：S852.7[农业科学—基础兽医学]