四川豇豆根腐病菌对咪鲜胺敏感基线的建立  

Baseline Sensitivity of Prochloraz to Cowpea Root Rot Fungus in Sichuan

作　　者：张河庆[1,2,3] 席亚东 韩帅[1,2,3] 吴婕 李洪浩[1] Zhang Heqing;Xi Yadong;Han Shuai;Wu Jie;Li Honghao(Institute of Plant Protection,Sichuan Academy of Agricultural Sciences,Chengdu 610066;Key Laboratory of Integrated Pest Management on Crops in Southwest,Ministry of Agriculture,Chengdu 610066;Vegetable Germplasm Innovation and Variety Improvement Key Laboratory of Sichuan Province,Chengdu 610066)

机构地区：[1]四川省农业科学院植物保护研究所,成都610066 [2]农业部西南作物有害生物综合治理重点实验室,成都610066 [3]蔬菜种质与品种创新四川省重点实验室,成都610066

出　　处：《中国农学通报》2018年第21期148-152,共5页Chinese Agricultural Science Bulletin

基　　金：四川省农作物抗病虫性鉴定技术创制与应用"蔬菜抗病性鉴定及监测"(2016NYZ0053-1-7);四川省科技支撑计划"现代生物技术及实用新技术集成研究与应用"(2016-XT00-00003-NC);四川省科技支撑计划"哈茨木霉与钩状木霉原生质体融合改良菌种的应用研究与示范"(2017RZ0027);现代农业产业技术体系四川蔬菜创新团队(nycytx-31);四川蔬菜花卉种植创新与产业提升关键技术研发专项"蔬菜抗病性评价与病原菌致病机理研究"(2017CYTS-018)

摘　　要：为评价四川豇豆根腐病菌对咪鲜胺的抗药性水平,科学使用咪鲜胺可持续防治豇豆根腐病,采用菌丝生长速率法,测定63株四川省豇豆根腐病菌对咪鲜胺的毒力曲线,建立敏感基线,评价其潜在的抗性风险。参试菌株的敏感性频率分布呈连续的单峰曲线,接近正态分布,平均值为EC50值(0.0063±0.0028)mg/L,这个值可用来监测豇豆根腐病原菌种群对咪鲜胺的敏感性变化。参试菌株中,61.9%为敏感菌株,38.1%为低抗药性菌株,没有出现高抗药性和中抗药性菌株。因此,只要加强田间抗药性监测,在四川豇豆生产上咪鲜胺仍可作为防治豇豆根腐病的有效药剂使用。To evaluate the resistance level of cowpea root rot fungus to prochloraz, and properly use prochloraz to control cowpea root rot, baseline sensitivity of prochloraz against 63 single spore Fusarium oxysporum isolated from cowpea in Sichuan was established with the mycelial growth inhibition method. The frequency distribution of the EC50 values of the tested isolates was a unimodal curve, with the mean EC_（50） value of（0.0063±0.0028） mg/L. The sensitivity data could be utilized as a baseline for monitoring the shift of sensitivity in the cowpea F. oxysporum isolates to prochloraz. Among the tested strains, 61.9% were susceptible strains, 38.1%were low-resistant strains, and no high-and mid-resistant strains were found. Therefore, as long as the field resistance monitoring is strengthened, prochloraz still can be used as a control agent for cowpea root rot in Sichuan.

关 键 词：豇豆根腐 咪鲜胺 敏感基线 

分 类 号：S482.2[农业科学—农药学]