从肉牛脂肪组织中提取总RNA技术方法的改进  

Improvement of Technique for Extracting Total RNA from Beef Adipose Tissue

作　　者：李娜[1,2] 余群力[1] 李红波[2] 闫向民[2] 张金山[2] 袁理星[2] 杜玮[2] 崔繁荣[2] 叶治兵 张杨 LI Na;YU Qun-li;LI Hong-bo;YAN Xiang-min;ZHANG Jin-shan;YUAN Li-xin;DU Wei;CUI Fan-rong;YE Zhi-bing;ZHANG Yang(Gansu Agricultural University,Lanzhou,730000;Institute of Animal Science,Xinjiang Academy of Animal Science,Urumqi,830000)

机构地区：[1]甘肃农业大学,兰州730000 [2]新疆畜牧科学院畜牧研究所,乌鲁木齐830000

出　　处：《中国牛业科学》2018年第3期8-11,共4页China Cattle Science

基　　金：现代农业产业技术体系建设专项资金资助(nycytx-37);自治区重点研发计划项目2017B01001

摘　　要：RNA提取技术是现代分子生物技术中一种常用技术。介绍一种高纯度、高产量的从动物组织中提取总RNA的改进的方法,该法实用性强、重复性好。提取的RNA无DNA等污染物,其产量、纯度完全能满足分子克隆和基因表达研究的需要。利用改进后的方法提取牛组织的总RNA,研究了Lep基因在牛组织中的表达分布。RNA extraction technology is a common technique in modern molecular biotechnology.This paper introduced an improved method of extracting total RNA with high purity and yield from animal tissues,which is practical and reproducible.And the extracted RNA without DNA and other pollutants,its yield and purity can fully meet the needs of the molecular cloning and gene expression research.The total RNA of bovine tissue was extracted by the improved method,and the expression of Lep gene in bovine tissue was studied.

关 键 词：RNA提取 脂肪组织 Lep基因 

分 类 号：S823[农业科学—畜牧学]