利用核心SNP标记划分辽宁省常用玉米自交系杂种优势群的研究  

Heterotic Grouping by Core SNP Markers for Maize Inbred Lines Widely Used in Liaoning Province

作　　者：姜思奇 郭瑞[1] 张敖 赵艳贺 时免免 邓丽霞 崔震海[1] 阮燕晔[1] JIANG Si-qi;GUO Rui;ZHANG Ao;ZHAO Yan-he;SHI Mian-mian;DENG Li-xia;CUI Zhen-hai;RUAN Yan-ye(College of Bioscience and Biotechnology,Shenyang Agricultural University,Shenyang 110000;Farming and Animal Husbandry Bureau,Beishan New District,Luanping County,Chengde 068250,China)

机构地区：[1]沈阳农业大学/辽宁省植物基因工程技术中心,沈阳110000 [2]河北省承德市滦平县北山新区农牧局,河北承德068250

出　　处：《玉米科学》2018年第4期17-23,共7页Journal of Maize Sciences

基　　金：辽宁省科技攻关项目“基于DOUGLAS基因型分析平台的玉米SNP标记体系的构建与优化”(2015103001)

摘　　要：利用3个密度的SNP标记对44份玉米自交系进行杂种优势群划分,包括8份对照自交系和36份辽宁省常用育种自交系。利用56k玉米芯片共筛选得到了46 899个高质量的SNP标记,作为高密度SNP标记划分杂种优势群,然后筛选1 008、101个SNP标记分别作为中密度和低密度SNP标记用于分群。结果表明,部分自交系的遗传相似度达到90%以上,不宜作为不同自交系进行育种应用。3个密度下的SNP标记都有效地将待测玉米自交系划分为4个杂种优势群,分别为瑞德群、兰卡斯特群、旅大红骨与塘四平头混合群以及类PH4CV群。对比高密度SNP标记,利用中低密度SNP标记划分的杂种优势群内部分自交系间的遗传距离发生变化,不能精确解析杂种优势群群内自交系的亲缘关系。划分杂种优势群可以采用中低密度的SNP育种芯片;群内自交系亲缘关系的区分应该采用高密度SNP芯片。In this study, 44 samples, including 8 representative inbred lines of classical heterosis groups and 36 popular inbred lines in maize breeding of Liaoning province, were grouped by three density groups of single nucleotide polymorphism（SNP）, respectively. A total of 46899 high-quality SNP markers obtained from 56k Maize Genome Array platform were used as the high-density group for heterosis grouping. Among them, 1008 and 101 core SNPs was selected as the medium-and low-density SNP groups for heterosis grouping, respectively. Then, some lines should not use as difference materials in breeding due to their genetic similarity was more than 90%. Cluster analysis with high-density SNP group classified the inbred lines into Reid, Lancaster, a mix group of Lvdahonggu and Tangsipingtou, and a like-PH4CV group. Similar results were both obtained in the cluster analysis with 1 008 and 101 core markers, however, the genetic distances between a few of the inbred lines changed. The results suggested that the low or medium-density SNP groups could economically and effectively separate heterosis groups, while the inbred lines relationships within group need the high-density SNPs group.

关 键 词：玉米 单核苷酸多态性 杂种优势群 遗传相似度 聚类分析 

分 类 号：S513.035.3[农业科学—作物学]