玉米弯孢叶斑病菌PG基因家族鉴定与表达分析  

Identification and Expression Analysis of PG Gene Family of Curvularia Leaf Spot

作　　者：刘震[1] 赵丰舟 崔佳[1] 曲建楠 刘铜 左豫虎[1] LIU Zhen;ZHAO Feng-zhou;CUI Jia;QU Jian-nan;LIU Tong;ZUO Yu-hu(College of Agronomy,Heilongjiang Bayi Agricultural University,Daqing 163319;College of Environment and Plant Protection,Hainan University,Haikou 570228,China)

机构地区：[1]黑龙江八一农垦大学农学院,黑龙江大庆163319 [2]海南大学热带农林科学院,海南海口570228

出　　处：《玉米科学》2018年第4期166-172,共7页Journal of Maize Sciences

基　　金：黑龙江八一农垦大学研究生创新项目(2011150043)

摘　　要：采用生物信息学鉴定Clpg基因家族成员,利用实时荧光定量PCR技术分析玉米弯孢叶斑病菌多聚半乳糖醛酸酶基因（Clpg）在病原菌-寄主植物互作时期的表达情况,鉴定Clpg基因的家族成员,研究每个成员在侵染过程的表达水平,明确Clpg基因在玉米弯孢叶斑病菌致病性中的作用。结果表明,玉米弯孢叶斑病菌Clpg基因家族有4个成员,分别命名为Clpg1、Clpg2、Clpg3和Clpg4,均含有3个内含子和2个外显子,与其他真菌PG基因具有相同的NTD、DD、GHG、RIK保守结构域。Clpg1基因的表达趋势为先升高后下降,在3 h达最高值;Clpg2、Clpg3和Clpg4基因的表达趋势为逐渐上升,结果暗示Clpg基因可能参与病原菌与寄主植物的互作过程。To uncover the role of polygalacturonase（Clpg） gene in Curvularia leaf spot of maize, the members of Clpg gene family were identified by bioinformatics analysis, and the expression level of each member during patho-gen-host interaction period was analyzed by real-time quantitative PCR. The results showed that Clpg gene familycontained four members, namely, Clpg1, Clpg2, Clpg3 and Clpg4. These Clpg genes all have the same exon and in-tron numbers and the same conserved domains（NTD, DD, GHG and RIK） as other fungi. The expression trends of Clpg1 genes first increased and then decreased, and reached the peak at 3h, while the expression trends of Clpg2,Clpg3 and Clpg4 genes increased gradually,which indicating that Clpg genes may play an important role in pathoge-nicity of Curvularia lunata（Walk） Boed in maize.

关 键 词：玉米 玉米弯孢叶斑病菌 Clpg基因 生物信息学分析 定量PCR 

分 类 号：S435.131[农业科学—农业昆虫与害虫防治]