dl-3-正丁基苯酞对内皮素诱导脑缺血大鼠行为学的改善研究  

Improvement of dl-3-n-butylphthalide on behavioral of rats with cerebral ischemia induced by endothelin

作　　者：张秀春[1] 程希 胡锦渠 钟姗珊 赵传胜[1] ZHANG Xiu-chun;CHENG Xi;HU Jin-qu;ZHONG Shan-shan;ZHAO Chuan-sheng(Department of Neurology,the First Hospital of China Medical University,Shenyang 110001,China;Department of Neurosurgery,the First Hospital of China Medical University,Shenyang 110001,China)

机构地区：[1]中国医科大学附属第一医院神经内科,辽宁沈阳110001 [2]中国医科大学附属第一医院神经外科,辽宁沈阳110001

出　　处：《现代药物与临床》2018年第7期1561-1565,共5页Drugs & Clinic

基　　金：国家自然科学基金资助项目(81372104);中国医科大学高层次创新团队培养计划项目(2017CXTD02)

摘　　要：目的探讨dl-3-正丁基苯酞对内皮素诱导脑缺血大鼠行为学的改善作用及其作用机制。方法采用向纹状体、皮层注射内皮素-1制作大鼠脑缺血模型。大鼠随机分为假手术组、模型组和dl-3-正丁基苯酞组。dl-3-正丁基苯酞组每日ig 70 mg/kg,起始于缺血后1周,持续给药2周。采用平衡木实验、粘性标签试验和圆筒试验评价神经功能恢复情况,应用免疫荧光技术观察海马齿状回新生神经细胞的情况。结果 dl-3-正丁基苯酞对脑缺血大鼠运功功能的恢复有明显的改善,明显促进海马齿状回的新生神经细胞增多,增加双皮质素(DCX)阳性细胞总树突长度。结论 dl-3-正丁基苯酞不仅能够对脑缺血后大鼠的运动功能有明显的改善,而且促进脑缺血后大鼠的神经再生,对脑缺血所致的神经细胞损坏的治疗提供参考。Objective To explore the improvement of dl-3-n-butyphthalide on behavioral in rats with cerebral ischemia and its mechanism. Methods Cerebral ischemia model in rats was made by intracerebral injection of endothelin-1. Rats were divided into Sham group, model group, and dl-3-n-butyphthalide group. Rats in dl-3-n-butyphthalide group were ig administered with dl-3-n-butyphthalide 70 mg/kg. It began at 1 week after ischemia, and continued to be administered for 2 weeks. Tapered/ledged beam-walking test, cylinder test, and sticky label test were used to evaluate the functional recovery. Dentate gyrus regenerative nerve cells were observed by immunohistochemistry. Results dl-3-n-Butyphthalide can obviously improve the function recovery of cerebral ischemia in rats, significantly increased the number of new neurons in the dentate gyrus of hippocampus, and increase the total dendrite length of DCX positive cells. Conclusion dl-3-n-Butyphthalide can not only improve the motor function of rats after cerebral ischemia, but also promote the regeneration of nerve in rats after cerebral ischemia, which may provide reference for the treatment of nerve cell damage caused by cerebral ischemia.

关 键 词：dl-3-正丁基苯酞 脑缺血 运动功能恢复 神经细胞再生 

分 类 号：R966[医药卫生—药理学]