新胃乃安片中4种异黄酮成分的同时鉴别及抗氧化活性的初步筛选  

Simultaneous identification of four isoflavones in Xinweinaian tablets and preliminary screening for antioxidant activities

作　　者：梁小银[1] 严萍[1] 詹若挺[1] 陈蔚文[1] LIANG Xiao-yin;YAN Ping;ZHAN Ruo-ting;CHEN Wei-wen(Ministry of Education Key Laboratol2~ of Chinese Medicinal Resource from Lingnan,Research Center of Chinese Herbal Resource Science and Engineering ＆ Joint Laboratory of National Engineering Research Center for the Pharmaceutics of Traditional Chinese Medicine,Guangzhou University of Chinese Medicine,Guangzhou 510006,Guangdong,China)

机构地区：[1]广州中医药大学中药资源科学与工程研究中心岭南中药资源教育部重点实验室(广州中医药大学)国家中成药技术研究中心南药研发实验室,广东广州510006

出　　处：《湖南中医杂志》2018年第7期186-187,206,共3页Hunan Journal of Traditional Chinese Medicine

基　　金：粤港关键领域重点突破招标项目(编号:GDDRC2007ZY002)

摘　　要：目的:建立新胃乃安片中芒柄花素、毛蕊异黄酮、芒柄花苷及毛蕊异黄酮苷4种异黄酮成分的同时鉴别方法,并对其抗氧化活性进行初步筛选研究。方法:采用薄层色谱-生物自显影技术,以甲苯-乙酸乙酯-甲醇(20∶20∶9)为展开剂对上述4种成分进行分离鉴别,以清除2,2-二苯基-1-苦肼基(DPPH)自由基能力对鉴定成分进行抗氧化活性筛选。结果:薄层色谱特征明显,在同一薄层板上能同时鉴别芒柄花素、毛蕊异黄酮、芒柄花苷及毛蕊异黄酮苷4种成分,其中筛选出毛蕊异黄酮和毛蕊异黄酮苷具有清除DPPH自由基的活性。结论:建立的方法操作简单,重现性好,可为新胃乃安片的质量控制及药效物质研究奠定基础。Objective： To investigate the method for the simultaneous identification of formononetin,calycosin,ononin,and calycosin-7-glucoside in Xinweinaian tablets and perform preliminary screening for antioxidant activities. Methods： With toluene-ethyl acetate-methanol（20∶ 20∶ 9） as the developing solvent,thin-layer chromatography-bioautography was performed for the isolation and identification of these four constituents,and their antioxidant activities were screened based on the ability to scavenge free radicals of 2,2-diphenyl-1-picryl-hydrazyl-hydrate（DPPH）. Results： There were significant features on thin-layer chromatography,and the simultaneous identification of formononetin,calycosin,ononin,and calycosin-7-glucoside was realized on the same thin-layer plate. Calycosin and calycosin-7-glucoside were found to have the activity to scavenge DPPH free radicals. Conclusion： The method established is simple and has good repeatability,and therefore,it lays a foundation for the quality control of Xinweinaian tablets and studies on effective constituents.

关 键 词：新胃乃安片 芒柄花素 毛蕊异黄酮 芒柄花苷 毛蕊异黄酮苷 实验研究 

分 类 号：R284.1[医药卫生—中药学]