兔骨髓源性单核细胞分化为血管内皮祖细胞的体外研究  

In vitro study of rabbit bone marrow-derived monocytes differentiation into vascular endothelial progenitor cells

作　　者：吕成鹏 李龙 倪华 刘斌 刘彦普 LYU Cheng-peng;LI Long;NI Hua;LIU Bin;LIU Yan-pu(Department of Stomatalogy,Shanxi Provincial People＇s Hospital,Xi＇an 710068,China)

机构地区：[1]陕西省人民医院口腔科,陕西西安710068 [2]空军军医大学口腔医院口腔颌面外科,陕西西安710032 [3]深圳市南山区蛇口人民医院口腔科,广东深圳51800 [4]空军军医大学口腔医院组织工程研发中心,陕西西安710032 [5]空军军医大学口腔医院动物实验中心,陕西西安710032

出　　处：《中国美容整形外科杂志》2018年第8期489-491,J0006,J0007,共5页Chinese Journal of Aesthetic and Plastic Surgery

基　　金：国家自然科学基金(81070820)

摘　　要：目的探讨采用密度梯度离心法和差速贴壁法分离兔骨髓源性血管内皮祖细胞,并对分离细胞进行培养及鉴定。方法通过细胞形态观察、免疫荧光染色、管腔形成实验以及低密度脂蛋白吞噬和植物凝集素贴附实验进行鉴定。结果分离培养的细胞呈多角形生长,细胞能够形成放射样克隆集落,细胞表达血管假性血友病因子(vW F),在基质胶呈管腔样生长,吞噬低密度脂蛋白并能够黏附植物凝集素。结论采用密度梯度离心法和差速贴壁法分离兔骨髓源性干细胞,在一定条件下能分化成为血管内皮祖细胞。Objective To explore the separation of rabbit bone marrow-derived vascular endothelial progenitor cells（EPCs） with the density gradient centrifugation and differential adhesion methods. Methods The separated cells were cultured and identified. EPCs were identified by general cellular morphologic observation, vW F immunofluorescence, lumen formation experiment, low density lipoprotein phagocytosis, and plant agglutinin attachment test. Results The EPCs grew as polygons, and could form clone colony-like radiation. The vW F was expressed in EPCs. The EPCs grew in the Matrigel as lumen, which can phagocytize low density lipoproteins and adhere to plant agglutinin. Conclusion It was concluded that the isolated rabbit bone marrow-derived monocytes cells which were separated through density gradient centrifugation and differential adhesion methods can successfully differentiate into endothelial progenitor cells under certain conditions.

关 键 词：血管内皮祖细胞 密度梯度离心 差速贴壁法 细胞分离 细胞培养 细胞鉴定 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]