HPLC法测定人血浆中伏立康唑的浓度  

Determination of the concentration of voriconazole in human plasma by HPLC method

作　　者：史长城[1,2,3] 楼江 王飞[1,2] 李晴宇 毛勇[1,2,3] 林能明 SHI Changcheng;LOU Jiang;WANG Fei;LI Qingyu;MAO Yong;LIN Nengming(Depart-ment of Pharmacy;Department of Clinical Pharmacy,Affiliated Hangzhou Hospital,Nanfing Medical University,Hangzhou 310006,China;Translational Medicine Research Center,Hangzhou First People＇ s Hospital,Hangzhou 310006,China)

机构地区：[1]杭州市第一人民医院药学部,杭州310006 [2]南京医科大学附属杭州医院临床药学研究室,杭州310006 [3]杭州市第一人民医院转化医学研究中心,杭州310006

出　　处：《中国临床药学杂志》2018年第4期235-238,共4页Chinese Journal of Clinical Pharmacy

基　　金：杭州市医学重点学科建设基金[编号:杭卫计发(2017)51号]

摘　　要：目的建立用于检测人血浆中伏立康唑浓度的HPLC法。方法以氯唑沙宗为内标,色谱柱为Agilent Zorbax Eclipse Plus C18(100 mm×4. 6 mm,3. 6μm);流动相为乙腈-0. 01 mol·L-1磷酸二氢钾溶液(35∶65);流速0. 8 m L·min-1;柱温35℃;检测波长260 nm。结果伏立康唑在质量浓度0. 2~20 mg·L-1内线性关系良好(r=0. 999 9),定量下限为0. 2mg·L-1,日间、日内精密度良好(RSD均<10%),低、中、高质量浓度下的相对回收率分别为104. 84%、99. 30%、100. 36%,提取回收率> 90%。结论本方法操作简便,结果准确,适用于人血浆中伏立康唑浓度的检测。AIM To establish an HPLC method for the determination of the concentration of voriconazole in human plasma. METHODS Chlorzoxazone was used as the internal standard. The analytical column was packed with Agilent Zorbax Eclipse Plus C ls （100mm×4.6mm,3.6μm） , the mobile phase was acetonitrile -0.01 mol · L-1 monopotassium phosphate （35：65） ,the flow rate was 0.8 mL ·min-1 ,the temperature was 35 ℃ and the de- tection wavelength was 260 nm. RESULTS The liner range of voriconazole in human plasma was 0.2 - 20 mg · L - 1 （ r = 0. 999 9）. The limit of quantitation was 0.2 mg · L - 1. The intra-day and inter-day RSD for assaying the plasma sample containing three concentration of voriconazole were both lower than 10%. The relative recovery was 104.84% , 99.30% and 100.36% respectively ,and the extraction recovery was more than 90%. CONCLUSION The method described is simple and accurate for the determination of the concentration of voriconazole in human plasma.

关 键 词：伏立康唑 高效液相色谱法 血药浓度 

分 类 号：R96[医药卫生—药理学] O657.72[医药卫生—药学]