2对同卵双生子microRNAs表达谱差异分析  

A preliminary study on the differential expression profile of microRNAs in two pairs of monozygotic twins

作　　者：肖超[1] 何华钰 刘尔亮 刘宇轩[4] 潘超[1] 易少华[1] 黄代新[1] Xiao Chao;He Huayu;Liu Ediang;Liu Yuxuan;Pan Chao;Yi Shaohua;Huang Daixini(Department of Forensic Medicine,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China;Xiaogan Municipal Public Security Bureau,Xiaogan 432000,China;Tianjin Municipal Public Security Bureau Wuqing Branch,Tianjin 301700,China;Suizhou Municipal Public Security Bureau,Suizhou 441300,China)

机构地区：[1]华中科技大学同济医学院法医学系,湖北武汉430030 [2]孝感市公安局,湖北孝感432000 [3]天津市公安局武清分局,天津301700 [4]随州市公安局,湖北随州441300

出　　处：《中国法医学杂志》2018年第3期241-247,共7页Chinese Journal of Forensic Medicine

基　　金：国家自然科学基金资助项目(81373250);湖北省自然科学基金资助项目(2013CFC084)

摘　　要：目的探索同卵双生子(monozygotic twins,MZ)间microRNAs(miRNAs)表达谱差异,寻找甄别MZ个体的新指标。方法应用miRNA芯片技术检测两对不同年龄和性别的MZ间miRNAs的表达并对其表达谱进行差异性分析,同时采用实时定量RT-PCR(qRT-PCR)技术对差异表达的miRNAs进行验证。结果男性MZ对共检出74个差异表达的miRNAs(51个上调,23个下调);女性MZ对共检出220个差异表达的miRNAs(102个上调,118个下调);两对MZ间共有的差异表达miRNAs 32个(上调27个,下调4个,表达不一致的1个)。此外,挑选的4个差异表达的miRNAs经实时qRT-PCR验证,与芯片检测结果一致。结论本研究初步证实了MZ个体间存在miRNAs表达差异,有望作为甄别MZ个体的新生物学指标。Objective To explore the differential expression of microRNAs （miRNAs） between monozygotic twins （MZ）, so as to to find new biomarkers for the individual identification of MZ. Methods The expression profiles of miRNAs of two pairs of MZ with different sex and age were detected by miRNA microarray, and then the expression difference was analyzed. The expression level of miRNAs was verified by real-time quantitative RT-PCR （qRT-PCR） technique. Results 74 miRNAs （51 up-regulated and 23 down-regulated） were differentially expressed between the male MZ, and 220 miRNAs （ 102 up- regulated and 118 down-regulated） were differentially expressed between the female MZ. Among these miRNAs, there were 32 miRNAs （27 up-regulated, 4 down-regulated and 1 inconsistent） which were differentially expressed in both of MZ pairs. In addition, the measurement results of the selected 4 differentially expressed miRNAs by real-time qRT-PCR were consistent with the results of microarray. Conclusion This study has preliminarily confirmed the difference of miRNAs expression between MZ, suggesting a new biological memaker in identifying MZ.

关 键 词：法医遗传学 同卵双生子 MICRORNAS 差异表达 

分 类 号：DF795.2[医药卫生—法医学]