宝塔红在过敏性猝死尸检中的应用初探  

The application of pagoda red in the sudden death of anaphylaxis

作　　者：谭晓辉 陈娜[2] 罗宝英 曾嘉敏 李佳 王慧君 Tan Xiaohui;Chen Na;Luo Baoying;Zeng Jiamin;Li Jia;Wang Huijun(Department of Forensic Science,Southern Medical University,Guangzhou 510515,China;Department of pathology,Guangdong Women and Children Hospital,Guang-zhou 511400,China)

机构地区：[1]南方医科大学法医学院,广东广州510515 [2]广东省妇幼保健院病理科,广东广州511400

出　　处：《中国法医学杂志》2018年第3期286-290,共5页Chinese Journal of Forensic Medicine

摘　　要：目的验证宝塔红染色法在过敏性猝死尸检中的应用可行性。方法选取我系历年过敏性猝死的案件30例,非过敏性死亡的案件30例,用1%宝塔红水溶液对上述案件脾脏组织的石蜡切片进行染色,显微镜下观察肥大细胞脱颗粒及嗜酸性粒细胞。结果过敏性猝死组:过敏性猝死组的脾切片均可看到脱颗粒的肥大细胞及显著增多的嗜酸性粒细胞。非过敏性猝死组:未见肥大细胞脱颗粒现象,部分案例散见的少量嗜酸性粒细胞。结论宝塔红染色效果稳定,受死后变化影响少,可作为过敏性猝死案件的辅助诊断指标。Objective application of allergic sudden 30 non-allergic sudden death Center of SMU. The paraffin Explore a simple, fast and accurate pagoda red staining method in the death autopsy. Methods Select 30 allergic sudden death autopsy samples and autopsy samples, all samples are from the cases in the Forensic Medical section of spleen tissue from above cases were stained with 1% pagoda red solution. Degranulated mastocytes and eosinophils were observed under microscopic. Results Degranulated mastocytes and eosinophils are seen in all samples from experimental group. A small amount of eosinophils without degranulated mastocytes are seen in all samples from control group. Conclusion Pagoda red dyeing effect is stable and reliable in results, compared with the conventional immunohistochemical staining method, less affected by the change after death, more suitable for judicial authentication autopsy. it may be a new method to test even diagnosis allergic sudden death. Accordingly.

关 键 词：过敏性猝死 宝塔红染色 肥大细胞脱颗粒 嗜酸性粒细胞 

分 类 号：F795.1[经济管理—产业经济]