不同滴度鸭肠炎病毒感染对鸭脾脏的转录组变化分析  

Transcriptome analysis of duck spleen infected with duck enteritis virus with different viral titers by high throughput

作　　者：吴良涛 郑敏[2] 华敏[2] 贺欣薇 万润[2] 程振涛 周碧君[2,3,4] 文明[2,3,4] WU Liang-tao;ZHENG Min;HUA Min;HE Xin-wei;WAN Run;CHENG Zhen-tao;ZHOUBi-jun;WEN Ming(Modern Terrace Agriculture Engineering Department,Qiannan Ethnic Vocational Technical College,Du yun,Guizhou 558022,China;College of Animal Sci-ence,Guizhou University,Guiyang 550025,China;Institute for Animal Diseases,Guizhou University,Guiyang 550025,China;Key Laboratory of Animal Diseases and Veterinary Public Health in Guizhou Province,Guiyang 550025,China)

机构地区：[1]黔南民族职业技术学院现代山地农业工程系,贵州都匀558022 [2]贵州大学动物科学学院,贵州贵阳550025 [3]贵州大学动物疫病研究所,贵州贵阳550025 [4]贵州省动物疫病与兽医公共卫生重点实验室,贵州贵阳550025

出　　处：《中国兽医学报》2018年第8期1487-1495,共9页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31260607,31560703);贵州省优秀青年科学人才培养计划资助项目(黔科合人字[2013]25号);贵州省百层次创新型人才资助项目(黔科合人才[2016]4009号);贵州省科技创新人才团队建设资助项目(黔科合人才团队[2015]4016号)

摘　　要：为探讨不同滴度鸭肠炎病毒（duck enteritis virus，DEV）感染对鸭组织器官转录组的影响，本研究采用2个不同DELD50滴度的DEV，接种50日龄健康鸭90h后，采集脾脏样本，高通量测序技术进行转录组测序，筛选差异表达基因，并应用GO与KEGG数据库进行分析。结果显示，当接种量为10^0xDELD50时，鸭脾脏差异表达基因有685个，其中上调基因341个，主要参与免疫反应、核糖体结构和酶活性等生物学过程，并在核糖体、氧化磷酸化和吞噬体信号通路中显著富集；TN基N为344个，主要参与细胞分化正调控、口转化生长因子生成正调控、酶与相关受体活性、细胞结构等生物学过程，并在细胞黏附分子、内吞作用、肠道免疫网络lgA产生、ECM受体互作、缝隙连接和黏着信号通路上显著富集。当接种量为10^0xDELD50时，鸭脾脏差异表达基因有485个，其中上调基因297个，主要参与细胞功能、蛋白结合和蛋白复合物等生物学过程，并在细胞黏附分子、吞噬体、肠道免疫网络IgA产生、球系列鞘糖脂生物合成及N-糖链合成信号通路中显著富集；下调基因188个，主要参与补体激活、肌肉组织活动调节、受体复合物、酶与受体活性等生物学过程，并在基础转录因子、PPAR信号通路、a-亚麻酸代谢等代谢途径与信号通路上显著富集。这些结果表明不同滴度DEV感染对鸭脾脏转录组产生不同的影响，为深入探究DEV致病分子机制提供基础资料。Abstract：To investigate the effects of duck enteritis virus （DEV） infection with different titers on the transcriptome of duck tissue, two LD50 titers of DEV were used to inoculate the 50 clay old healthy ducks,and the spleen samples of ducks were collected at 90 h inoculation for the transcrip- tome sequencing by high-throughput technology and the differentially expressed genes were ana- lyzed by GO and KEGG bioinformatics. The results showed as the follows：when the inoculation a- mount was 10^0 XDELD50 of DEV,there were 685 of differentially expressed genes in duck spleen, among which 341 were up-regulated genes, mainly involved in immune response,ribosome struc- ture and enzyme activity and other biological processes, which significantly enriched in the ribosome,oxidative phosphorylation and phagosome signal pathways. And 344 were down-regulated genes, mainly involved in positive regulation of cell differentiation and transforming growth factor beta production, enzyme and receptor activity, cell structure and other biological processes, which sig- nificantly enriched in cell adhesion molecules, endocytosis,intestinal immune network for IgA pro- duction, ECM receptor interaction, gap junction and focal adhesion signal pathways. When the inoc- ulation amount was 10^-2 X DELD50 of DEV,there were 485 of differentially expressed genes induck spleen, among which 297 were up-regulated genes, mainly involved in cell function, protein binding,protein complexes and other biological processes,which significantly enriched in phago- some, cell adhesion molecules,intestinal immune network for IgA production, glycosphingolipid biosynthesis-globo series and N-glycan biosynthesis. And 188 were down-regulated genes, mainly involved in complement activation, muscle activity regulation, receptor complexes, enzyme and receptor activity and other biological processes, which significantly enriched in basal transcription fac tors,PPAR signaling pathway and alpha linolenic acid metabolism. These results indicated that the DEV

关 键 词：病毒滴度 鸭肠炎病毒 脾脏 转录组 高通量测序技术 

分 类 号：S858.325[农业科学—临床兽医学] S852.65[农业科学—兽医学]