Wnt/β-catenin通路在脉冲强磁场促神经干细胞增殖中的作用初探  

Role of Wnt/β-catenin pathway in proliferation of neural stem cells induced by high-intensity pulsed electromagnetic fields

作　　者：龙兴蓝 许涛[2] 邹丽丽[3] 蒋婷 彭涛[5] 孙衢骎 Long XingZan;Xu Tao;Zou LiZi(Department of Rehabilitation Medicine,Three Gorges University People＇s Hospital,Yichang 443000,China)

机构地区：[1]三峡大学人民医院康复医学科,湖北宜昌443000 [2]华中科技大学同济医学院附属同济医院康复医学科,武汉430030 [3]河南省人民医院康复医学科,郑州450000 [4]武汉大学中南医院康复医学科,武汉430030 [5]华中科技大学脉冲强磁场中心,武汉430030

出　　处：《中国康复》2018年第4期267-271,共5页Chinese Journal of Rehabilitation

基　　金：国家自然科学基金(面上项目)(81271732)

摘　　要：目的:摸索抑制神经干细胞(NSCs)中β-catenin表达的合适条件,为获得抑制β-catenin表达的NSCs做准备。利用抑制β-catenin表达的NSCs,探索wnt/β-catenin通路在脉冲强磁场促NSCs增殖中的作用。方法:取新生的SD大鼠双侧侧脑室下的NSCs,然后用无血清培养基培养14d。利用RNAi原理构建β-catenin shRNA慢病毒载体及阴性慢病毒载体,并转染NSCs,确定合适的感染复数(MOI)值。将NSCs分为阴性组(阴性病毒转染组)和实验组(β-catenin shRNA病毒转染组),并用4T、0.1Hz、8次的脉冲强磁场干预。在用脉冲强磁场干预后的1d、7d,采用CCK8法检测阴性组和实验组的NSCs增殖情况。结果:β-catenin shRNA序列在MOI=10时,对NSCs中β-catenin的抑制效果最好。阴性病毒组在脉冲强磁场干预后1d、7d时NSCs增殖最明显(P<0.05),而实验组在干预前后NSCs增殖水平无明显变化。结论:wnt/β-catenin通路在脉冲强磁场促NSCs增殖中起作用。Objective：To investigate the optimal conditions of inhibiting theβ-catenin expression in neural stem cells（NSCs）for harvesting NSCs low-expressingβ-catenin,and exploring the roles of Wnt/β-catenin pathway in proliferation of NSCs induced by the high-intensity pulsed electromagnetic fields.Methods：（1）NSCs were derived from subventricular zone（SVZ）of newborn SD rats,and then cultured for 14 days using serum-free medium.（2）The RNAi method was used to inhibitβ-catenin expression in NSCs,and the optimal MOI for transfection to inhibitβ-catenin levels in NSCs was measured.（3）The NSCs were divided into the negative control group and experimental group,and then they were exposed in the high-intensity pulsed electromagnetic fields with stimulus condition of 4 T,0.1 Hz,8 times.（4）At 1 st and 7 th day after the intervention with the high-intensity pulsed electromagnetic fields,the proliferation of NSCs was tested by the CCK8 assay.Results：（1）When MOI=10,the best inhibitory effects onβ-catenin could be obtained in NSCs.（2）At 1 st and 7 th day after NSCs were exposed to the high-intensity pulsed electromagnetic fields,the proliferation of NSCs in the negative control group was significantly higher than in the experimental group（P〈0.05）.Conclusion：The Wnt/β-catenin pathway may play a role in proliferation of NSCs induced by the high-intensity pulsed electromagnetic fields.

关 键 词：神经干细胞 脉冲强磁场 增殖 RNAI WNT/Β-CATENIN通路 

分 类 号：R49[医药卫生—康复医学] R329.2[医药卫生—临床医学]