一测多评法同时测定常春藤口服液中4种皂苷  

Simultaneous determination of four saponins in Changchunteng Oral Liquid by QAMS

作　　者：张立国[1] 王晓敏[1] 孙磊 栾绍嵘[1] 倪力军[1] 金汉台 朱美鹏 金志文 ZHANG Li-guo;WANG Xiao-min;SUN Lei;LUAN Shao-rong;NI Li-jun;JIN Han-tai;ZHU Mei-peng(School of Chemistry and Molecular Engineering,East China University of Science and Technology,Shanghai 200237,China;Zhejiang CONBA Chinese Medicine Co.,Ltd.,Songyang 323400,China)

机构地区：[1]华东理工大学化学与分子工程学院,上海200237 [2]浙江康恩贝中药有限公司,浙江松阳323400

出　　处：《中成药》2018年第8期1756-1762,共7页Chinese Traditional Patent Medicine

基　　金：国家重大新药创制项目(2017ZX09201008009-105)

摘　　要：目的建立一测多评法同时测定常春藤口服液(常春藤)中4种成分的含有量。方法该药物甲醇提取液的分析采用Waters XTERRA RP-C18色谱柱(250 mm×4.6 mm,5μm);流动相水-乙腈,梯度洗脱;柱温25℃;体积流量1.0 m L/min;检测波长205 nm。以常春藤皂苷C为内标,计算其他3种成分的相对校正因子,测定其含有量。结果常春藤皂苷C、常春藤皂苷D、常春藤皂苷B、α-常春藤皂苷分别在0.009 5~0.191 0、0.001 7~0.033 1、0.001 2~0.024 1、0.001 9~0.038 6 mg/m L范围内线性关系良好(r≥0.999 8),平均加样回收率(RSD)分别为98.97%(1.12%)、99.83%(1.37%)、98.47%(1.19%)、99.57%(1.82%)。一测多评法所得结果与外标法接近。结论该方法稳定可靠,可用于常春藤口服液的质量控制。AIM To establish a quantitative analysis of multi-components by single-marker（ QAMS） method for the simultaneous content determination of four saponins in Changchunteng Oral Liquid（ Hedera helix L.）.METHODS The analysis of methanol extract of this drug was performed on a 25 ℃ thermostatic Waters XTERRA RP-C18 column（ 250 mm × 4. 6 mm,5 μm）,with the mobile phase comprising of water-acetonitrile flowing at1. 0 m L/min in a gradient elution manner,and the detection wavelength was set at 205 nm. With hederacoside C as an internal standard,the relative correction factors of the other three constituents were calculated,after which the content determination was made. RESULTS Hederacoside C,hederacoside D,hederasaponin B,α-hederin showed good linear relationships within the ranges of 0. 009 5-0. 191 0,0. 001 7-0. 033 1,0. 001 2-0. 024 1,0. 001 9-0. 038 6 mg/m L（ r ≥ 0. 999 8）,whose average recoveries（ RSDs） were 98. 97%（ 1. 12%）,99. 83%（ 1. 37%）,98. 47%（ 1. 19%）,99. 57%（ 1. 82%）,respectively. The results obtained by QAMS approximated those obtained by external standard method. CONCLUSION This stable and reliable method can be used for the quality control of Changchunteng Oral Liquid.

关 键 词：常春藤口服液 常春藤皂苷C 常春藤皂苷D 常春藤皂苷B α-常春藤皂苷 一测多评 

分 类 号：R927.2[医药卫生—药学]