二乙烯亚胺对水貂肠炎细小病毒灭活效果的研究  

Inactivation of Mink Enteritis Virus with Binary Ethylenimine

作　　者：罗国良[1] 王振军[1] 冯二凯[1] 易立[1] 郭利[1] 叶明 张淼 程悦宁[1] LUO Guo-liang;WANG Zhen-jun;FENG Er-kai;YI Li;GUO Li;YE Ming;ZHANG Miao;CHENG Yue-ning(Institute of Special Wild Economic Animals and Plants,Chinese Academy of Agricultural Sciences,Changchun 130112,China;Jilin Teyan Biotechnology Company Limited,Changchun 130122,China)

机构地区：[1]中国农业科学院特产研究所,长春130112 [2]吉林特研生物技术有限责任公司,长春130122

出　　处：《特产研究》2018年第3期23-26,共4页Special Wild Economic Animal and Plant Research

基　　金：吉林省科技发展计划项目(20150307003NY)

摘　　要：为研究二乙烯亚胺(BEI)对水貂肠炎细小病毒的灭活效果,采用BEI终浓度为0.003mol/L、0.002mol/L、0.001mol/L、0.0005mol/L,灭活温度为30℃,灭活时间为24h、48h、72h条件下,对水貂肠炎细小病毒进行灭活试验。通过F81细胞传代观察细胞病变和血凝试验检测灭活效果;用水貂检验BEI灭活水貂肠炎细小病毒所制疫苗的安全性,通过检测接种水貂的血凝抑制抗体,评价BEI灭活细小病毒所制疫苗的免疫效果,同时与甲醛灭活的疫苗进行免疫效果比较。结果表明,BEI终浓度0.002mol/L、30℃24h是灭活水貂肠炎细小病毒的适宜参数,BEI灭活工艺制备的水貂肠炎细小病毒灭活疫苗具有良好的安全性,BEI灭活工艺与甲醛灭活工艺的免疫抗体水平差异不显著(P>0.05)。本研究为水貂肠炎细小病毒灭活工艺的研究提供了理论依据。To observe the inactivation effect of binary ethylenimine（BEI） on mink enteritis virus（MEV）, the mink enteritis virus was inactivated 24h, 48h and 72h after treatment with different concentration ofBEI（0.003mol/L, 0.002mol/L, 0.001mol/L, 0.000 5mol/L） at 30℃ o The assurance for BEI inactivation was performed by cytopathic effect（CPE） in F81 cell cultures and hemagglutination（HA） test. The minks were used to test the safety of the BEI-inactivated vaccine. The hemagglutination inhibition antibody from mink were used to evaluate the immune efficacy of the two kinds of vaccine, which were inactivated with BEI andformaldehyde. The results indicated that the suitable parameters of MEV inactivated by BEI is 0.002mol/L ofBEI, at 30℃, for 24h. The vaccine inactivate with BEI was safe to the mink, and made a slightly higher HI antibody lever than the formaldehyde-inactivated vaccine. This study provides references for re- search of mink enteritis virus inactivated vaccine.

关 键 词：水貂肠炎细小病毒 二乙烯亚胺 灭活效果 

分 类 号：S852.655[农业科学—基础兽医学]