普通烟草Nt4CL基因家族的生物信息学分析  

Bioinformatics Analysis on Tobacco Nt4CL Gene Family

作　　者：陈夏晔 彭宣翔 CHEN Xiaye, PENG Xuanxiang(Chenzhou Branch Company of Hunan Tobacco Company ,Chenzhou, Hanan 423000 China)

机构地区：[1]湖南省烟草公司郴州市公司,湖南郴州423000

出　　处：《贵州农业科学》2018年第8期11-15,共5页Guizhou Agricultural Sciences

摘　　要：为烟草分子育种提供参考,利用序列相似性比对鉴定烟草中的20个Nt4CL基因,并对其基因结构、蛋白理化性质及保守结构域、亚细胞定位进行分析预测。基于普通烟草栽培品种K326转录组数据,对20个Nt4CL基因在烟叶不同生长发育时期的表达量进行可视化分析。结果表明:烟草中Nt4CL基因数目庞大,但其蛋白结构、理化性质等高度保守,且各个家族成员存在组织特异性表达;选择烟草叶片高表达的Nt4CL基因进行功能研究,可为烟草木质素合成的分子调控研究奠定基础。In order to provide references for molecular breeding of tobacco, the authors identified 20 Nt4CL genes in tobacco genome, and analyzed gene structure, protein physicochemical property, protein conserved domains and subcellular localization for these genes. What＇s more,based on K326 transcriptome data,the expression pattern for the 20 Nt4CL genes were visualized in leaves of different developmental stages. Analysis results show that the tobacco Nt4CL genes are in large numbers, hut their protein structure, physico chemical properties are highly conserved, and there are histologically specific expressions for each family member. Functional analysis of Nt4CL genes with high expression levels in leaves will lay the foundation for molecular regulation of tobacco lignin synthesis.

关 键 词：木质素 香豆酸 辅酶A连接酶 烟草 基因家旋 

分 类 号：S572[农业科学—烟草工业]