一例猪伪狂犬病野毒感染的实验室诊断  

Laboratory Diagnosis on a Case of Infection with Wild Type Pseudorabies Virus

作　　者：黎露 韩涛涛 吴思雯 唐青海 杨海 何丽芳 刘最 王芳宇 张倩 Li Lu;Han Taotao;Wu Siwen;Tang Qinghai;Yang Hai;He Lifang;Liu Zui;Wang Fangyu;Zhang Qian(Key laboratory of biotechnology,College of Life Science and Environment,Hengyang Normal University,Hengyang,Hunan421008,China)

机构地区：[1]衡阳师范学院生命科学与环境学院生物技术重点实验室,湖南衡阳421008

出　　处：《中国动物检疫》2018年第9期77-80,共4页China Animal Health Inspection

基　　金：国家自然基金青年基金(31101837);衡阳师范学院引进人才专项(16D20);湖南省教育厅优秀青年项目(17B041)

摘　　要：为确诊衡阳市某猪场育肥猪发生的疑似猪伪狂犬病(PR)疫情,采集发病猪脾脏,运用PCR方法进行猪伪狂犬病病毒(PRV)核酸检测,利用非洲绿猴肾细胞(Vero)进行病毒分离培养,采用免疫过氧化物酶单层细胞染色法(IPMA)进行病毒血清学鉴定;用分离培养物进行家兔感染试验,观察临床症状和病例剖检变化,采用PCR检测各器官中的病毒分布情况。结果显示:病料接种Vero细胞培养3 d后,出现明显的细胞病变效应(CPE);分离培养的病毒与PRV阳性血清呈阳性反应;家兔感染后出现奇痒等神经症状并死亡,其肝、脾组织均为PRV gE核酸检测阳性。试验结果证实,分离到的病毒为PRV野毒,从而确诊了该疑似疫情。In order to identify the suspected porcine pseudorabies（PR）epidemic in fattening pigs in a farm of Hengyang City,the spleens of infected pigs were collected and porcine pseudorabies virus（PRV）nucleic acids were detected by PCR method. Then,virus isolation and serological identification were carried out by Vero cells and immunoperoxidase mono-layer assay（IPMA）. The rabbits were infected with the isolated culture and clinical symptoms and necropsy changes were observed. Finally,the virus distribution situation in different organs was determined by PCR. Results showed that,after being inoculated with tissue samples for 3 days,the Vero cells showed significant cytopathic effect（CPE）. The results of IPMA showed positive reaction between isolated virus and PRV-positive serum. The rabbits appeared itchy and other neurological symptoms and died,and PRV gE nucleic acids were detected in the liver and spleen tissues of these rabbits. As a conclusion,the epidemic was caused by PRV infection,and the isolated virus was wild PRV.

关 键 词：猪伪狂犬病毒 PCR 分离培养 免疫过氧化物酶单层细胞染色法 

分 类 号：S852.65[农业科学—基础兽医学]