脑梗死患者外周血中Cx40基因多态性与粥样斑块性质发展变化的相关性  

Correlation between peripheral blood Cx40 gene polymorphism and atherosclerotic plaque property development in patients with cerebral infarction

作　　者：李毓新[1] 周胜[2] LI Yu-xin;ZHOU Sheng(Neurology Department No.1,the Second Affiliated Hospital of Xi’an Medical University,Xi’an710038,China;Department of Anorectum ,the Second Affiliated Hospital of Xi＇an Medical University,Xi＇an710038,China)

机构地区：[1]西安医学院第二附属医院神经内一科,陕西西安710038 [2]西安医学院第二附属医院肛肠科,陕西西安710038

出　　处：《海南医学院学报》2018年第16期1475-1478,共4页Journal of Hainan Medical University

基　　金：陕西省自然科学基金资助项目(2012JM4005)~~

摘　　要：目的:研究脑梗死患者外周血中缝隙连接蛋白40(Cx40)基因多态性与粥样斑块性质发展变化的相关性。方法:选择2015年3月~2018年3月期间因急性脑梗死在西安医学院第二附属医院就诊的患者作为脑梗死组,选择同期体检的健康者作为对照组。采集外周血并测定Cx40基因rs35594137位点的多态性,采集血清并测定细胞因子、蛋白酶及其相关分子的含量。结果:脑梗死组患者外周血中Cx40基因AA+AG基因型的构成比高于对照组,GG基因型的构成比低于对照组;脑梗死组患者血清中IL-17、HMGB1、VCAM1、MCP-1、P-selectin、YKL-40、MMP9、TIMP1、Caspase-3的含量以及MMP9/TIMP1的比值均显著高于对照组,ADAMTS13、Vaspin的含量均显著低于对照组;脑梗死组中CX40基因AA+AG基因型患者血清中IL-17、HMGB1、VCAM1、MCP-1、P-selectin、YKL-40、MMP9、TIMP1、Caspase-3的含量以及MMP9/TIMP1的比值均显著高于GG基因型患者,ADAMTS13、Vaspin的含量均显著低于GG基因型患者。结论:脑梗死患者外周血中Cx40基因rs35594137位点等位基因G向A突变会促进粥样斑块性质的发展变化。Objective:To study the correlation between peripheral blood connexin 40 (Cx40) gene polymorphism and atherosclerotic plaque property development in patients with cerebral infarction．Methods:Patients who were treated in the Second Affiliated Hospital of Xi’an Medical University due to acute cerebral infarction between March 2015 and March 2018 were selected as cerebral infarction group,and healthy subjects who received physical examination during the same period were selected as control group．Peripheral blood was collected to detect the polymorphism of Cx40 gene rs35594137 locus,and serum was collected to determine the contents of cytokines,proteases and related molecules．Results:The constituent ratio of Cx40 gene AA＋AG genotype inperipheral blood of cerebral infarction group was higher than that of control group whereas the constituent ratio of GG genotype was lower than that of control group;serumIL-17,HMGB1,VCAM1,MCPＧ1,P-selectin,YKL-40,MMP9,TIMP1 and Caspase-3 contents as well as MMP9/TIMP1 ratio of cerebral infarction group were significantly higher than those of control group whereas ADAMTS13 and Vaspin contents were significantly lower than those of control group;serumIL-17,HMGB1,VCAM1,MCP-1,P-selectin,YKL-40,MMP9,TIMP1 and Caspase-3 contents as well as MMP9/TIMP1 ratio of cerebral infarction group of patients with CX40 gene AA＋AG genotype were significantly higher than those of patients with GG genotype whereas DAMTS13 and Vaspin contents were significantly lower than those of patients with GG genotype (P ＜0.05)．Conclusion:The mutation from Cx40 gene rs35594137 allele G to A in peripheral blood of pa-tients with cerebral infarction can promote the development of atherosclerotic plaque properties．

关 键 词：脑梗死 缝隙连接蛋白40 基因多态性 动脉粥样斑块 

分 类 号：R563.9[医药卫生—呼吸系统]