肝郁脾虚型慢性乙型肝炎患者Th1/Th2类细胞功能研究  

Study on the Function of Th1/Th2 Types Cell in Chronic Hepatitis B Patients with Symdrome of Stagnation of Liver Qi with Deficiency of Spleen Postgraduate

作　　者：谢维丽 王少扬[2] 刘海周[2] 谢志红[2] XIE Weili;WANG Shaoyang;LIUHaizhou l(Haicang Hospital ofXiamen,Xiamen City,Fujian Province 361000)

机构地区：[1]厦门海沧医院,福建省厦门市361000 [2]南京军区福州总医院感染科

出　　处：《医学理论与实践》2018年第16期2386-2388,共3页The Journal of Medical Theory and Practice

摘　　要：目的:研究肝郁脾虚型慢性乙型肝炎患者Th1/Th2类细胞功能,为慢性乙型肝炎中医证型微观辨证提供相关依据,从而从免疫学角度为中医治疗慢性乙型肝炎提供理论依据。方法:选择20例肝郁脾虚型HBeAg阴性慢性乙型肝炎患者为观察对象,同时选择10例湿热中阻型HBeAg阳性慢性乙型肝炎患者和10例肝郁脾虚型HBeAg阳性慢性乙型肝炎患者为观察对象,10例正常人群为正常对照组,通过细胞内染色技术,采用流式细胞仪检测外周血CD3^+CD8^-T细胞内IFN-γ和IL-4表达,反映Th1、Th2类细胞功能。结果:(1)与正常对照组比较,3组中医辨证分型慢性乙肝组外周血IFN-γ百分比、IFN-γ/IL-4比值均明显降低,IL-4百分比均明显升高;湿热中阻型HBeAg阳性慢性乙肝组、肝郁脾虚型HBeAg阳性慢性乙肝组外周血IFN-γ百分比、IFN-γ/IL-4比值差异无统计学意义,而肝郁脾虚型HBeAg阴性慢性乙肝组外周血IFN-γ百分比、IFN-γ/IL-4均明显降低。(2)3组中医辨证分型慢性乙肝外周血IL-4百分比的差异均无统计学意义。结论:慢性乙型肝炎患者存在Th1/Th2类细胞失衡,表现为Th2类细胞功能优势表达;肝郁脾虚型慢性乙肝患者,就HBeAg阴性和HBeAg阳性患者比较,后者分泌较高水平的IFN-γ,表现为不同的免疫应答效应;HBeAg阳性慢性乙型肝炎肝郁脾虚型与湿热中阻型相比,Th1和Th2类细胞功能没有明显差异。Objective： To study the function of Th1/Th2 types cell in hepatitis B patients with syndrome of stagnation of liver qi with deficiency of spleen postgraduate,which can provide related basis for micro syndrome differentiation in chronic hepatitis B patients,and theoretical basis for traditional chinese medicine in treating patients with chronic hepatitis B from the immunological view of point. Methods： 20 HBeAg-negative and 10 HBeAg-positive chronic hepatitis B patients with syndrome of stagnation of liver qi with deficiency of spleen and 10 HBeAg-positive chronic hepatitis B patients with syndrome of[accumulated]damp-heat were selected as study subjects,6 healthy subjects as control group. The levels of the cytokine produced by CD4 cell were measured by flow cytometry. Results： There was difference in IFN-γ between HBeAg-positive and HBeAg-negative chronic hepatitis B patients with symdrome of stagnation of liver qi with deficiency of spleen（ P 0. 05）; however,there was no difference in IFN-γbetween HBeAg-positive chronic hepatitis B patients with symdrome of stagnation of liver qi with deficiency of spleen and symdrome of[accumulated]damp-heat（ P 0. 05）; there was no significant difference in the levels of IL-4 and IFN-γ/IL-4 among HBeAg-negative chronic hepatitis B patients with symdrome of stagnation of liver qi with deficiency of spleen,HBeAg-positive chronic hepatitis B patients with symdrome of stagnation of liver qi with deficiency of spleen and that with symdrome of[accumulated]damp-heat（ P 0. 05） ： there was significant difference in expression of IFN-γ,IL-4 and IFN-γ/IL-4 between the experimental group and the control group,especially in the difference of level of IL4 and IFN-γ/IL-4（ P 0. 05）. Conclusion： There was significant difference between healthy subjects and patients with chronic hepatitis B in Th1/Th2 imbalance and the latter expressed Th2 function obviously; patients with HBeAg-negative chronic hepatitis B and that with HBeAg-positive chronic hepatitis B have Im

关 键 词：乙型肝炎 肝郁脾虚型 TH1/TH2 流式细胞术 

分 类 号：R574.1[医药卫生—消化系统]