L-茶氨酸对氧化应激断奶仔猪生长性能及血液生化指标的影响  

Effects of L-theanine on Growth Performance and Blood Biochemical Indicators of Oxidative Stress Weaned Piglets

作　　者：闫峻[1,2] 李泽青 王文杰 刘通[4] 马衍旋[4] 李宁 马墉[1] 郑梓[1] 穆淑琴[1] YAN Jun1,2 , LI Zeqing3 , WANG Wenjie1 , LIU Tong4 , MA Yanxuan4 , LI Ning1 , MA Yong1 , ZHENG Zi1 , MU Shuqin1(1. Tianjin lnstitttte of Animal Science and Veterinary, Tianjin Academy of Agricltural Science, Tianjin 300381, China; 2. College of Chemical Engineering, Tianjin University, Tianjin 300072, China; 3. Tianjin Animal Husbandry and Veterinary Station, Tianjin 301800, China; 4. College of Animal Science and Technology, Northwest A＆F University, Yangling 712100, Chin)

机构地区：[1]天津市农业科学院,天津市畜牧兽医研究所,天津300381 [2]天津大学化工学院,天津300381 [3]天津市畜牧兽医站,天津301800 [4]西北农林科技大学动物科技学院,杨凌712100

出　　处：《中国畜牧兽医》2018年第8期2175-2181,共7页China Animal Husbandry & Veterinary Medicine

基　　金：天津市生猪产业技术体系创新团队(ITTPRS2017005); 天津市农科院院长基金(15001); 天津市科技支撑计划项目(15ZCZDNC00090)

摘　　要：本研究探讨了L-茶氨酸对氧化应激断奶仔猪生长性能、抗氧化能力及免疫功能的影响。试验采用2×2二因素设计,160头断奶仔猪(7.53kg±0.51kg)随机分为4个处理组:1组(日粮中不添加L-茶氨酸,腹腔注射5mL生理盐水);2组(日粮中不添加L-茶氨酸,腹腔注射8 mg/kg体重敌快死);3组(日粮中添加1 000mg/kg L-茶氨酸,腹腔注射5mL生理盐水);4组(日粮中添加1 000mg/kg L-茶氨酸,腹腔注射8mg/kg体重敌快死)。预试期7d,正试期28d。结果显示:(1)与1组相比,2组仔猪平均日增重(ADG)和平均日采食量(ADFI)显著降低,而料重比显著升高(P<0.05);与2组相比,4组仔猪ADG显著增加(P<0.05)。(2)与1组相比,2组仔猪血清丙二醛(MDA)含量显著增加,而总抗氧化能力(T-AOC)和谷胱甘肽过氧化物酶(GSH-Px)活性显著下降(P<0.05),3组仔猪血清MDA显著降低,而T-AOC显著增加(P<0.05);与2组相比,4组仔猪血清MDA含量显著降低,而T-AOC和GSH-Px活性显著上升(P<0.05)。(3)与1组相比,2组仔猪血清IgA、IgG、IL-2、IL-4水平显著增加,C3和C4水平显著下降(P<0.05),3组仔猪血清IgA、IgG和IL-4水平显著降低,而C3和C4水平显著增加(P<0.05);与2组相比,4组仔猪血清IgA、IgG、IL-2、IL-4水平显著降低,而血清C3水平显著增加(P<0.05)。结果表明,注射敌快死可引发仔猪氧化应激,造成生长性能下降、体液免疫能力下降;日粮中添加1 000mg/kg L-茶氨酸可缓解仔猪氧化损伤,提高氧化应激仔猪的生长性能、抗氧化能力和体液免疫能力。To explore the effects of L-theanine on growth performance,antioxidant and immune response of oxidative stress weaned pigs,a two-factor（2×2）experimental design was employed.160 weaned pigs（7.53 kg±0.51 kg）were divided into 4 treatment groups：Treatment 1（withoutL-theanine,intraperitoneal injection of 5 mL normal saline）,treatment 2（without L-theanine,intraperitoneal injection of 8 mg/（kg·BW）diquat）,treatment 3（diet added 1 000 mg/kg L-theanine,intraperitoneal injection of 5 mL normal saline）and treatment 4（diet added 1 000 mg/kg Ltheanine,intraperitoneal injection of 8 mg/（kg·BW）diquat）.7 dfor preliminary feeding and 28 d for formal feeding.The results showed that：（1） Compared to treatment 1,the average daily gain（ADG）and average daily feed intake（ADFI）of weaned pigs in treatment 2 decreased significantly（P〈0.05）,while F/G increased significantly（P〈0.05）;Compared to treatment 2,L-theanine significantly increased ADG and ADFI in treatment 4（P〈0.05）.（2）Compared to treatment 1,diquat significantly increased serum malondialdehyde（MDA）content,while the total antioxidant capacity（T-AOC）and the activity of glutathione peroxidase（GSH-Px）significantly decreased in treatment 2（P〈0.05）,serum MDA content in treatment 3 significantly increased while the activity of GSH-Px significantly tended down（P〈0.05）;Compared to treatment 2,L-theanine significantly decreased serum MDA content while increased the activity of T-AOC and GSH-Px in treatment 4（P〈0.05）.（3）Compared to treatment 1,the levels of serum IgA,IgG and IL-2,IL-4 significantly increased,while C3 and C4 significantly decreased（P〈0.05）in treatment 2,and the levels of serum IgA,IgG and IL-4 were significantly decreased,while the concentration of C3 and C4 significantly increased in treatment 3（P〈0.05）;Compared to treatment 2,the levels of serum IgA,IgG and IL-2,IL-4 significantly decreased,while C3 significantly increased in treatment 4（P〈0.05）.In co

关 键 词：L-茶氨酸 断奶仔猪 氧化应激 生长性能 体液免疫 

分 类 号：S816.7[农业科学—饲料科学]