超滤法结合大孔吸附树脂法制备复方双中药注射液  

Preparation of Compound Double Traditional Chinese Medicine Injection by Ultrafiltration Method and Macroporous Adsorption Resin Method

作　　者：罗甜[1] 肖新荣[1] 黄卓贤 苗青 LUO Tian,XIAO Xinrong ,HUANG Zhuoxian,MIAO Qing(School of Chemistry and Chemical Engineering,University of South China,Hengyang,Hunan 421001,Chin)

机构地区：[1]南华大学化学化工学院,湖南衡阳421001

出　　处：《南华大学学报（自然科学版）》2018年第4期90-96,共7页Journal of University of South China：Science and Technology

基　　金：湖南省科技计划资助项目(2014FJ3134)

摘　　要：超滤法初步提取双中药银杏叶和绞股蓝中抗心脑血管疾病有效成分,利用AB-8大孔吸附树脂对初提物中银杏黄酮、绞股蓝黄酮、绞股蓝皂苷等进行进一步纯化,制备出复方银杏绞股蓝双中药盐酸曲美他嗪注射液.同时,进行了双中药注射液稳定性研究.研究结果表明,在超声振荡的条件下,超滤法提取抗心脑血管疾病有效成分正交实验的最佳条件为:乙醇体积浓度80%,固液比1∶20,超声时间10 min.AB-8大孔吸附树脂纯化的最佳条件为:上样液浓度总黄酮为1.252 3 mg/mL^2.504 7mg/mL,总皂苷为0.429 4 mg/mL^1.288 3 mg/mL,洗脱剂为体积浓度为70%的乙醇.纯化后产物中总黄酮、总皂苷的平均质量分数分别为37.35%、12.75%,黄酮与皂苷的比值为2.93.注射剂稳定性考察结果显示在30 d内-10℃、25℃条件下有效成分的含量变化在5%以内.The ultrafiltration method was used to extract anti-cardiovascular and cerebrovascular diseases active ingredients of ginkgo biloba and gynostemma pentaphyllum.The active ingredients of ginkgo flavonoids,gynostemma flavonoids,and gynostemma saponin were further purified by AB-8 macroporous adsorption resin.The purpose of the study was to prepare the compound ginkgo gynostemma pentaphyllum double traditional chinese medicine trimetazidine hydrochloride injection and investigate its stability.Results show that in the condition of ultrasonic oscillation,the optimum conditions for the ultrafiltration extraction of active ingredients in ginkgo biloba and gynostemma pentaphyllum were as follows： with 80% ethanol concentration,the ratio of solid to liquid was 1∶20,and the ultrasonic time was 10 min.The optimum purification conditions for AB-8 macroporous adsorption resin were as follows： The concentration of total flavonoids in the sample solution was 1.252 3 mg/mL-2.504 7 mg/mL,the total saponin was 0.429 4 mg/mL-1.288 3 mg/mL,the eluent solution was 70% ethanol.The purity of total flavonoids and total saponins from purification were 37.35%,12.75%,and the ratio of flavonoids and saponins was 2.93.The results of the injection stability shows that the content of the active ingredient changes within 5% for 30 days at -10 ℃ and 25 ℃.

关 键 词：银杏叶 绞股蓝 超滤 AB-8大孔吸附树脂 注射液 

分 类 号：TQ469[化学工程—制药化工]