干旱胁迫对杜鹃叶片表皮解剖结构的影响  

Effect of Drought Stress on Leaf Epidermal Anatomical Structure of Rhododendron

作　　者：周媛 方林川 童俊 董艳芳 徐冬云 毛静 魏鸣 ZHOU Yuan1,FANG Lin-chuan1,TONG Jun1,DONG Yan-fang1,XU Dong-yun1,MAO Jing1,WEI Ming2(1.Institute of Forestry ＆ Fruit Tree,Wuhan Academy of Agricultural Science,Wuhan 430075,China;2.Wuhan Academy of Agricultural Science,Wuhan 430065,Chin)

机构地区：[1]武汉市农业科学院林业果树研究所,武汉430075 [2]武汉市农业科学院,武汉430065

出　　处：《湖北农业科学》2018年第14期67-72,共6页Hubei Agricultural Sciences

基　　金：武汉市农业科学院创新团队项目(CX2018);国家自然科学基金青年科学基金项目(31300587)

摘　　要：以杜鹃品种胭脂蜜[Rhododendron obtusum(Lindl.)Planch.cv.Yanzhimi]、花蝴蝶(R.obtusum cv.Huahudie)和西施(R.obtusum cv.Xishi)为研究试材,在光照培养箱内采用盆栽控水法进行干旱胁迫,利用石蜡切片与扫描电子显微镜观测叶片的解剖结构。结果表明,杜鹃叶片仅有下表皮具有气孔,上、下表皮都有表皮毛和腺毛等附属物,随干旱胁迫程度的加重,气孔开度变小,到中后期气孔闭合。叶片受干旱后变薄,栅栏组织/海绵组织之比随干旱胁迫时间的延长而增大,其中胭脂蜜的栅栏组织/海绵组织之比变化幅度最大,并且抗旱性较强的胭脂蜜气孔的闭合时间相对更晚,说明叶片细胞结构对于杜鹃抗旱性强弱的决定作用较大。Using paraffin section and scanning electron microscope,the leaf anatomical structure were investigated in Rhododendron obtusum（Lindl.） Planch. cv. Yanzhimi,R. obtusum cv. Huahudie and R. obtusum cv. Xishi under drought stress in light incubator. The results showed that the stomata only distributed in the lower epidermis and there were epidermal hair and trichome on both upper and lower epidermis. With heavier drought stress,the width of stomata became smaller and then the stomata closed in the later period. The leaf was thinner and the ratio of the palisade tissue to spongy tissue increased after drought stress. The change of ratio of palisade tissue to spongy tissue was the largest, and the time of stomata closure was relatively later in R. obtusum cv. Yanzhimi with relatively highest drought resistance. There is an important association between leaf anatomical structure and drought resistance in Rhododendron.

关 键 词：杜鹃[Rhododendron obtusum(Lindl.)Planch.] 干旱胁迫 抗旱性 叶片解剖结构 扫描电子显微镜 

分 类 号：S685.21[农业科学—观赏园艺] Q948.112.33[农业科学—园艺学]