八个不同产地绞股蓝总皂苷含量及抗氧化活性测定  

Determination of Total Saponins and Antioxidant Activity of Gynostemma pentaphyllum(Thunb) Makino from Eight Different Producing Areas

作　　者：马泽刚 黄春花 钟辉云 吴秀丽 周礼仕 刘卓[2] MA Ze-gang1, HUANG Chun- hua1, ZHONG Hui- yun1, WU Xiu-li1, ZHOU Li- shi1, LIU Zhuo2(l.Dcpartmcnt of Pharmacy,Sichuan Vocational College of Health and Rehabilitation,Zigong 621000,Sichuan,China;2.School of Life Science and Engineering,Southwest University of Science and Technology,Mianyang 621000,Sichuan,Chin)

机构地区：[1]四川卫生康复职业学院药学系,四川自贡643000 [2]西南科技大学生命科学与工程学院,四川绵阳621000

出　　处：《湖北农业科学》2018年第14期109-113,共5页Hubei Agricultural Sciences

基　　金：四川省教育厅自然科学基金项目(17ZB0366);四川卫生康复职业学院校级课题项目(CWKY-2016-04)

摘　　要：建立绞股蓝[Gynostemma pentaphyllum(Thunb)Makino]总皂苷的最佳提取工艺条件,并比较不同产地绞股蓝中的总皂苷含量以及抗氧化活性差异。结果表明,绞股蓝总皂苷最佳提取工艺为60℃条件下,60%乙醇回流提取4次,提取时间2 h,料液比为1∶10。不同产地绞股蓝总皂苷含量为江西>广东>云南>陕西>内蒙古>福建>四川>湖北。DPPH自由基清除率结果表明,不同产地绞股蓝抗氧化能力为陕西>湖北>江西>内蒙古>广东>福建>云南>四川。建立的绞股蓝中总皂苷含量提取工艺简便、稳定、可行,不同产地绞股蓝中总皂苷含量和抗氧化活性存在差异,该结果可为今后绞股蓝的开发利用和深入研究提供一定的参考。To establish optimal extraction technology for the total saponins in Gynostemma pentaphyllum,and prepare the difference of content from different producing areas,and the difference of antioxidant activity. Results showed that the optimal extraction technology was as follow：under 60 ℃,Gynostemma pentaphyllum powder was by reflux extraction in 60% ethanol for 4 times with each time for 2 h. The ratio of sample to extraction solution 1∶10. Total saponin content of Gynostemma pentaphyllum from different producing areas was difference. The highest was Jiangxi,and the lowest was Hubei. The results of DPPH scavenging rate showed that the antioxidant activity of Gynostemma pentaphyllum from different producing areas was difference.The highest was Shaanxi,and the lowest was Sichuan. The results showed that the extraction process was simply,stable and feasible. There was a significant difference in the content of total saponin and antioxidant activity in Gynostemma pentaphyllum from the different areas. It provided certain reference significance for development and further study of the resources in Gynostemma pentaphyllum.

关 键 词：绞股蓝[Gynostemma pentaphyllum(Thunb)Makino] 总皂苷 抗氧化 提取工艺 

分 类 号：R284[医药卫生—中药学]