安徽省西瓜枯萎病菌遗传多样性ISSR-PCR分析  

Genetic Diversity of Fusarium oxysporum f. sp. neviumin Anhui based on ISSR-PCR Markers

作　　者：李萍[1] 刘冬[1,2] 姜晓斌[1] 余碧霞 LI Ping1, LIU Dong1,2 , JIANG Xiao-bin1, YU Bi-xia 1(1. Department of Horticulture and Landscape, Anqing Vocational and Technical College, Anqing 246003,China; 2. School of Plant Protection, Anhui Agricultural University, Hefei 230036, Chin)

机构地区：[1]安庆职业技术学院园林园艺系,安徽安庆246003 [2]安徽农业大学植物保护学院,安徽合肥230036

出　　处：《牡丹江师范学院学报（自然科学版）》2018年第3期58-61,共4页Journal of Mudanjiang Normal University：Natural Sciences Edition

基　　金：安徽省高校自然科学研究项目(KJ2015A368;KJ2013B133);安徽省高校优秀青年人才支持计划重点项目(gxyqZD2016516;gxyqZD2018120);安徽省质量工程教学改革项目(2016jyxm0639);安庆职业技术学院中青年骨干教师项目

摘　　要：以42株来自安徽省不同地区的西瓜枯萎病菌为试材,采用Inter-simple Sequence Repeats(ISSR)技术对其群体的遗传多样性进行分析,探究病菌的遗传分化与地理来源之间的相关性.结果表明,用筛选出的11个随机引物对西瓜枯萎病菌菌株进行ISSR-PCR扩增,共获得129个ISSR条带,其中多态性条带为94个,占72.9%,表明安徽省西瓜枯萎病菌存在较丰富的遗传多样性,菌株间的遗传相似系数为0.71~0.97.UPGMA聚类分析显示,供试菌株可区分为8个聚类组,菌株的遗传谱系与地理来源无相关性.In order to probe into the relationship between genetic diversity and geographical origin among isolates of Fusarium oxysporumf.sp.neviumfrom Anhui Province in eastern China,inter-simple sequence repeat-polymerase chain reaction（ISSR-PCR）was conducted to investigate the genetic diversity of 42 isolates of F.oxysporum f.sp.nevium.The result showed that 11 random primers which produced reproducible fragments were screened,generated a total of 129 ISSR fragments,of which 72.9%（94）were polymorphic,revealing high polymorphism among the isolates.Genetic similarity coefficients among all of the isolates ranged from 0.71 to 0.97.Cluster analysis using the unweighted pair-group method with arithmetic averages（UPGMA）indicated that the Anhui isolates were divided into eight groups according to the DNA fingerprints,and that no correlation between ISSR group and geographical origin.

关 键 词：西瓜枯萎病菌(Fusarium oxysporumf. sp. niveum) ISSR-PCR 遗传多样性 

分 类 号：S436.5[农业科学—农业昆虫与害虫防治]