两种差示分光光度法测定血清苯巴比妥浓度比较  

Determination of phenobarbital in serum by two methods of differential spectrophotometry and their comparison

作　　者：王刚[1] 谷容[1] 刘彬[1] 张振琪[1] 

机构地区：[1]重庆医科大学附属儿童医院,400014

出　　处：《儿科药学》2002年第3期30-33,共4页Journal of Pediatric Pharmacy

摘　　要：目的:建立两种条件下差示分光光度法测定血清苯巴比妥浓度的方法,比较其回收率、RSD、稳定性和信号响应等。方法:在酸性条件下以三氯甲烷从血清中二次提取,利用苯巴比妥在强碱与弱感,碱性与酸性介质中吸收度差值与浓度有良好的线性,分别按不同条件下的差示分光光度法测定血清中苯巴比妥浓度。结果:两种方法血清苯巴比妥浓度的线性范围2～80μg/ml,在弱碱与酸性的条件下,其回收率和RSD分别为100.66％±0.36％、2.17％±0.08％,血清苯巴比妥在此条件下25min内较稳定,对测定结果无显著影响（P>0.05）。在强碱与弱碱性的条件下,回收率和RSD分别是（99.98±0.89）％和（4.73±0.18）％,血清中提取的苯巴比妥在10min时存在显著性影响（P<0.01）,15min吸收度较最初降低9％。结论:按弱碱与酸性条件下的差示分光光度法测定血清苯巴比妥,有较好的稳定性和准确性。Objective: To develop two methods for determining the contents of phenobarbital in serum by differential spectrophotometry under two conditions,to comparison the average recovery,BSD,stability and noise of signal of determination et. Methods: Phenobarbital in serum was extracted two times with trichloromethane under acidic condition. The standard curves of Phenobarbital in serum were established according to the absor-bance deviations of phenobarbital in strong base and weak base, weak base and acidic media, and the phenobarbital in serum was determined by differential spectraphotometry under differential conditions.Results: The linear range of phenobarbital in serum under two conditions were 2-80 μg/ml,the average recovery and BSD were 100.66%±0.36% ,2.17%±0.08% under weak base and acidic condition; and the result of determination of phenobarbital in serum needless to be influenced in 25 min (P>0.05) .The average recovery and RSD were (99.98±0.89) % , (4.73 ±0.18) % under strong and weak base condition, the result of determination of phenobarbital in serum seriously was influenced in 10 min (P < 0.001),the absorbance of phenobarbital is lower 9% than original.Conclusions: The result determined of phenobarbital in serum by differential spectrophotomatry under weak base and acidic conditions has better stability and accuracy than strong and weak base.

关 键 词：苯巴比妥 差示分光光度法 血清浓度 稳定性 

分 类 号：R969.1[医药卫生—药理学]